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Characterization of a human cDNA that encodes a functional receptor for platelet activating factor
Ye R.D.; Prossnitz E.R.; Zou A.; Cochrane C.G.
1991-10-15
Source PublicationBiochemical and Biophysical Research Communications
ISSN10902104 0006291X
Volume180Issue:1Pages:105-111
AbstractWe have cloned a cDNA for the platelet activating factor (PAF) receptor by screening an HL-60 granulocyte cDNA library with degenerate oligonucleotide probes based on conserved sequences of rhodopsin-type receptors. The 342-amino acid receptor contains 7 putative transmembrane domains, but lacks sites for N-linked glycosylation at the N-terminus. Stably transfected fibroblasts expressing the cloned cDNA responded to sub-nanomolar PAF stimulation with calcium mobilization, which could be inhibited by the PAF antagonist L659,989. The PAF receptor message was detected as a single species of approximately 4 kb in human placenta, lung, and differentiated HL-60 granulocytes. Expression of the cloned cDNA in undifferentiated HL-60 cells devoid of endogenous PAF receptor resulted in specific and saturable binding of the PAF antagonist WEB 2086 with a dissociation constant of 30.7 nM. © 1991 Academic Press, Inc.
KeywordG proteins guanine nucleotide regulatory proteins PAF platelet activating factor
DOI10.1016/S0006-291X(05)81261-6
URLView the original
Language英語
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Document TypeJournal article
CollectionPersonal research not belonging to the institution
AffiliationScripps Research Institute
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GB/T 7714
Ye R.D.,Prossnitz E.R.,Zou A.,et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor[J]. Biochemical and Biophysical Research Communications,1991,180(1):105-111.
APA Ye R.D.,Prossnitz E.R.,Zou A.,&Cochrane C.G..(1991).Characterization of a human cDNA that encodes a functional receptor for platelet activating factor.Biochemical and Biophysical Research Communications,180(1),105-111.
MLA Ye R.D.,et al."Characterization of a human cDNA that encodes a functional receptor for platelet activating factor".Biochemical and Biophysical Research Communications 180.1(1991):105-111.
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