UM
Cell type- and developmental stage-specific activation of NF-κB by fMet-Leu-Phe in myeloid cells
Browning D.D.; Pan Z.K.; Prossnitz E.R.; Ye R.D.
1997-04-08
Source PublicationJournal of Biological Chemistry
ISSN00219258
Volume272Issue:12Pages:7995-8001
AbstractChemoattractants induce a variety of phagocytic functions including transendothelial migration, degranulation, and the generation of superoxide anions. We report here that the prototypic chemotactic peptide fMet-Leu-Phe (fMLF) stimulates the activation of nuclear factor-κB (NF-κB), a transcription factor that is central to the regulation of proinflammatory immediate-early gene expression. In freshly prepared peripheral blood mononuclear cells, fMLF (1-100 nM) induced a κB binding activity that was receptor-dependent and involved the p50 and p65 subunits of the NF-κB/Rel family of proteins. The activation of NF-κB by fMLF appeared to be cell- specific and different from the activation of NF-κB by tumor necrosis factor-α (TNFα). Neutrophil preparations that responded to fMLF, TNFα, and lipopolysaccharides with interleukin-8 secretion did not show NF-κB activation, whereas N-formyl peptide receptor (FPR)-transfected HL-60 cells were responsive to TNFα but not fMLF for NF-κB activation. Differentiation of FPR-transfected HL-60 cells with dimethyl sulfoxide for 3-5 days conferred the capability of the cells to activate NF-κB in response to fMLF without a significant increase in the amount of FPR. These results identify NF-κB as a transcription factor that can be activated by the prototypic chemotactic peptide and demonstrate that this function is both highly regulated and dependent on signaling components specifically expressed during myeloid differentiation.
DOI10.1074/jbc.272.12.7995
URLView the original
Language英語
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Cited Times [WOS]:59   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationScripps Research Institute
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GB/T 7714
Browning D.D.,Pan Z.K.,Prossnitz E.R.,et al. Cell type- and developmental stage-specific activation of NF-κB by fMet-Leu-Phe in myeloid cells[J]. Journal of Biological Chemistry,1997,272(12):7995-8001.
APA Browning D.D.,Pan Z.K.,Prossnitz E.R.,&Ye R.D..(1997).Cell type- and developmental stage-specific activation of NF-κB by fMet-Leu-Phe in myeloid cells.Journal of Biological Chemistry,272(12),7995-8001.
MLA Browning D.D.,et al."Cell type- and developmental stage-specific activation of NF-κB by fMet-Leu-Phe in myeloid cells".Journal of Biological Chemistry 272.12(1997):7995-8001.
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