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Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis
Le Y.1; Ye R.D.3; Gong W.1; Li J.1; Iribarren P.1; Wang J.M.1
2005-02-01
Source PublicationFEBS Journal
ISSN1742464X 17424658
Volume272Issue:3Pages:769-778
AbstractFormyl peptide receptor-like 1 (FPRL1) is a seven transmembrane domain, G protein-coupled receptor that interacts with a variety of exogenous and host-derived agonists. In order to identify domains crucial for ligand recognition by FPRL1, we used chimeric receptors with segments in FPRL1 replaced by corresponding amino acid sequences derived from the prototype formyl peptide receptor FPR. The chimeric receptors were stably transfected into human embryonic kidney epithelial cells and the capacity of the cells to migrate in response to formyl peptide receptor agonists was evaluated. Our results showed that multiple domains in FPRL1 are involved in the receptor response to chemotactic agonists with the sixth transmembrane domain and the third extracellular loop playing a prominent role. Interestingly, the N-terminus and a segment between the fourth transmembrane domain and the third intracellular loop of FPRL1 are important for receptor interaction with a 42 amino acid amyloid âpeptide (Aβ), an Alzheimer's disease-associated FPRL1 agonist, but not with MMK-1, a synthetic FPRL1 agonist, suggesting that diverse agonists may use different domains in FPRL1. Considering the potential importance of FPRL1 in inflammation and neurodegenerative diseases, the identification of functional domains in this receptor will provide valuable information for the design of specific receptor antagonists.
KeywordChemotaxis Formyl peptide receptor Formyl peptide receptor-like 1 Structure-function
DOI10.1111/j.1742-4658.2004.04514.x
URLView the original
Language英語
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Cited Times [WOS]:29   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.National Cancer Institute at Frederick
2.Shanghai Institute for Biological Sciences Chinese Academy of Sciences
3.University of Illinois College of Medicine
Recommended Citation
GB/T 7714
Le Y.,Ye R.D.,Gong W.,et al. Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis[J]. FEBS Journal,2005,272(3):769-778.
APA Le Y.,Ye R.D.,Gong W.,Li J.,Iribarren P.,&Wang J.M..(2005).Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis.FEBS Journal,272(3),769-778.
MLA Le Y.,et al."Identification of functional domains in the formyl peptide receptor-like 1 for agonist-induced cell chemotaxis".FEBS Journal 272.3(2005):769-778.
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