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Stereospecific induction of nuclear factor-κB activation by isochamaejasmin
Tian Q.2; Li J.3; Xie X.4; Sun M.4; Sang H.3; Zhou C.4; An T.4; Hu L.4; Ye R.D.3; Wang M.-W.4
2005-12-01
Source PublicationMolecular Pharmacology
ISSN0026895X 15210111
Volume68Issue:6Pages:1534-1542
AbstractThe root of Stellera chamaejasme L. is a traditional Chinese herb termed Rui Xiang Lang Du and has been used to treat solid tumors, tuberculosis and psoriasis. Exactly how S. chamaejasme L. regulates cellular responses remains unclear. We examined four biflavonoids isolated from S. chamaejasme L., including isochamaejasmin, two of its stereo-isomers and a methyl derivative, in functional assays originally designed to screen ligands for the G protein-coupled formyl peptide receptor-like 1 (FPRL1). Isochamaejasmin was found to induce the expression of a nuclear factor (NF)-κB-directed reporter gene in transfected HeLa cells with an EC of 3.23 μM, independently of FPRL1. The isochamaejasmin-stimulated NF-κB reporter activity was accompanied by nuclear translocation of NF-κB proteins and was blocked by a dominant-negative construct of IκBα. Isochamaejasmin also induced time-dependent phosphorylation of the mitogen-activated protein kinases extracellular signal-regulated kinase 1/2 and p38, and a novel protein kinase C (PKCδ). Likewise, inhibition of these kinases with the respective pharmacological inhibitors significantly reduced the isochamaejasmin-stimulated NF-κB activation. It is noteworthy that the two stereoisomers and the methyl derivative did not induce detectable activation of NF-κB and were more cytotoxic than isochamaejasmin, which could partially rescue cycloheximide-induced apoptosis. Inhibition of NF-κB activation reversed the anti-apoptotic effect of isochamaejasmin. These results provide the first evidence for a potential mechanism of action by S. chamaejasme L., and indicate that structurally similar compounds derived from S. chamaejasme L. may have different pharmacological properties. Copyright © 2005 The American Society for Pharmacology and Experimental Therapeutics.
DOI10.1124/mol.105.014720
URLView the original
Language英語
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.National Center for Drug Screening
2.Chinese Academy of Sciences
3.University of Illinois College of Medicine
4.Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Tian Q.,Li J.,Xie X.,et al. Stereospecific induction of nuclear factor-κB activation by isochamaejasmin[J]. Molecular Pharmacology,2005,68(6):1534-1542.
APA Tian Q..,Li J..,Xie X..,Sun M..,Sang H..,...&Wang M.-W..(2005).Stereospecific induction of nuclear factor-κB activation by isochamaejasmin.Molecular Pharmacology,68(6),1534-1542.
MLA Tian Q.,et al."Stereospecific induction of nuclear factor-κB activation by isochamaejasmin".Molecular Pharmacology 68.6(2005):1534-1542.
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