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Lipopolysaccharide stimulates platelet secretion and potentiates platelet aggregation via TLR4/MyD88 and the cGMP-dependent protein kinase pathway
Zhang G.1; Han J.1; Welch E.J.1; Ye R.D.1; Voyno-Yasenetskaya T.A.1; Malik A.B.1; Du X.1; Li Z.1
2009-06-15
Source PublicationJournal of Immunology
ISSN00221767 15506606
Volume182Issue:12Pages:7997-8004
AbstractBacterial LPS induces rapid thrombocytopenia, hypotension, and sepsis. Although growing evidence suggests that platelet activation plays a critical role in LPS-induced thrombocytopenia and tissue damage, the mechanism of LPS-mediated platelet activation is unclear. In this study, we show that LPS stimulates platelet secretion of dense and α granules as indicated by ATP release and P-selectin expression, and thus enhances platelet activation induced by low concentrations of platelet agonists. Platelets express components of the LPS receptor-signaling complex, including TLR (TLR4), CD14, MD2, and MyD88, and the effect of LPS on platelet activation was abolished by an anti-TLR4-blocking Ab or TLR4 knockout, suggesting that the effect of LPS on platelet aggregation requires the TLR4 pathway. Furthermore, LPS-potentiated thrombin- and collagen-induced platelet aggregation and FeCl-induced thrombus formation were abolished in MyD88 knockout mice. LPS also induced cGMP elevation and the stimulatory effect of LPS on platelet aggregation was abolished by inhibitors of NO synthase and the cGMP-dependent protein kinase (PKG). LPS-induced cGMP elevation was inhibited by an anti-TLR4 Ab or by TLR4 deficiency, suggesting that activation of the cGMP/protein kinaseGpathway by LPS involves the TLR4 pathway. Taken together, our data indicate that LPS stimulates platelet secretion and potentiates platelet aggregation through a TLR4/MyD88- and cGMP/PKG-dependent pathway. Copyright © 2009 by The American Association of Immunologists, Inc.
DOI10.4049/jimmunol.0802884
URLView the original
Language英語
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Cited Times [WOS]:208   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.University of Illinois College of Medicine
2.University of Kentucky HealthCare
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GB/T 7714
Zhang G.,Han J.,Welch E.J.,et al. Lipopolysaccharide stimulates platelet secretion and potentiates platelet aggregation via TLR4/MyD88 and the cGMP-dependent protein kinase pathway[J]. Journal of Immunology,2009,182(12):7997-8004.
APA Zhang G..,Han J..,Welch E.J..,Ye R.D..,Voyno-Yasenetskaya T.A..,...&Li Z..(2009).Lipopolysaccharide stimulates platelet secretion and potentiates platelet aggregation via TLR4/MyD88 and the cGMP-dependent protein kinase pathway.Journal of Immunology,182(12),7997-8004.
MLA Zhang G.,et al."Lipopolysaccharide stimulates platelet secretion and potentiates platelet aggregation via TLR4/MyD88 and the cGMP-dependent protein kinase pathway".Journal of Immunology 182.12(2009):7997-8004.
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