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Characterization of Quin-C1 for its anti-inflammatory property in a mouse model of bleomycin-induced lung injury
He M.2; Cheng N.1; Gao W.-W.2; Zhang M.2; Zhang Y.-Y.2; Ye R.D.1; Wang M.-W.2
2011-05-01
Source PublicationActa Pharmacologica Sinica
ISSN16714083 17457254
Volume32Issue:5Pages:601-610
AbstractAim: To study the in vivo effects of Quin-C1, a highly specific agonist for formyl peptide receptor 2 (FPR2/ALX), in a mouse model of bleomycin (BLM)-induced lung injury. Methods: Male ICR mice were injected intratracheally with BLM (d 0), and intraperitoneally with Quin-C1 (0.2 mg/d) or vehicle between d 1 and d 28, during which pulmonary inflammation was monitored. A similar regimen was carried out between d 5 and d 28 to differentiate anti-inflammatory from anti-fibrotic effects. During the treatment, leukocyte numbers in bronchoalveolar lavage fluid (BALF) were counted, and FPR2/ALX transcripts, tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), the mouse keratinocyte-derived chemokine (KC), transforming growth factor β1 (TGF-β1) and C-X-C motif chemokine 10 (CXCL10) expression levels in the lung tissue were also measured. Both hydroxyproline content and histological changes were examined on d 28 to assess the severity of lung fibrosis. Results: BLM caused a significant increase in expression levels of all the selected cytokines and chemokines, as well as a thickening of the alveolar wall. Treatment with Quin-C1 significantly reduced the neutrophil and lymphocyte counts in BALF, diminished expression of TNF-α, IL-1β, KC, and TGF-β1, and decreased collagen deposition in lung tissue. The treatment also lowered the content of lung hydroxyproline. Quin-C1 did not ameliorate lung fibrosis when the treatment was started 5 d after the BLM challenge, suggesting that the protection may be attributed to its anti-inflammatory effects. Exposure to BLM or BLM plus Quin-C1 did not change the level of FPR2/ALX transcripts (mFpr1, mFpr2, and Lxa4r) in the lung tissue. Conclusion: The results demonstrate an anti-inflammatory role for Quin-C1 in bleomycin-induced lung injury, which may be further explored for therapeutic applications. © 2011 CPS and SIMM All rights reserved.
Keywordanti-inflammation bleomycin cytokines formyl peptide receptor lung injury Quin-C1
DOI10.1038/aps.2011.4
URLView the original
Language英語
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.University of Illinois College of Medicine
2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences
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GB/T 7714
He M.,Cheng N.,Gao W.-W.,et al. Characterization of Quin-C1 for its anti-inflammatory property in a mouse model of bleomycin-induced lung injury[J]. Acta Pharmacologica Sinica,2011,32(5):601-610.
APA He M..,Cheng N..,Gao W.-W..,Zhang M..,Zhang Y.-Y..,...&Wang M.-W..(2011).Characterization of Quin-C1 for its anti-inflammatory property in a mouse model of bleomycin-induced lung injury.Acta Pharmacologica Sinica,32(5),601-610.
MLA He M.,et al."Characterization of Quin-C1 for its anti-inflammatory property in a mouse model of bleomycin-induced lung injury".Acta Pharmacologica Sinica 32.5(2011):601-610.
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