UM
Application of nickel (II) thermo-responsive affinity polymer to porcine circovirus type 2 (PCV2) cap protein purification and interaction analysis by X-ray photoelectron spectroscopy (XPS)
Li, Sipeng; Liu, Jifu; Ding, Zhaoyang; Cao, Xuejun
2018-06
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume69Pages:216-223
AbstractThe vaccine against porcine circovirus type II (PCV2) is mainly composed of virus capsid proteins. An efficient separation method of PCV2 Cap protein has not been developed. Affinity precipitation methods with high selectivity and recoverability based on the thermo-responsive affinity polymer (P-NDBN-Ni) were introduced to separate the PCV2 Cap protein. When the temperature was above the polymer LCST (36.1 degrees C), P-NDBN-Ni could be precipitated with a recovery of above 95.00%. The affinity ligand immobilized on the polymer could specifically bind the proteins. The optimal conditions for affinity precipitation were a pH of 6.0 with a ligand density of 56.22 mu mol/g polymer at 25.0 degrees C. The final results were detected by electrophoresis. The interactions between proteins and ligands were determined by the change in ligand state as seen using X-ray photoelectron spectra (XPS).
KeywordPorcine circovirus type II capsid protein Affinity precipitation Thermo-responsive X-ray photoelectron spectroscopy
DOI10.1016/j.procbio.2018.03.007
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000435749200024
PublisherELSEVIER SCI LTD
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Li, Sipeng,Liu, Jifu,Ding, Zhaoyang,et al. Application of nickel (II) thermo-responsive affinity polymer to porcine circovirus type 2 (PCV2) cap protein purification and interaction analysis by X-ray photoelectron spectroscopy (XPS)[J]. PROCESS BIOCHEMISTRY,2018,69:216-223.
APA Li, Sipeng,Liu, Jifu,Ding, Zhaoyang,&Cao, Xuejun.(2018).Application of nickel (II) thermo-responsive affinity polymer to porcine circovirus type 2 (PCV2) cap protein purification and interaction analysis by X-ray photoelectron spectroscopy (XPS).PROCESS BIOCHEMISTRY,69,216-223.
MLA Li, Sipeng,et al."Application of nickel (II) thermo-responsive affinity polymer to porcine circovirus type 2 (PCV2) cap protein purification and interaction analysis by X-ray photoelectron spectroscopy (XPS)".PROCESS BIOCHEMISTRY 69(2018):216-223.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Sipeng]'s Articles
[Liu, Jifu]'s Articles
[Ding, Zhaoyang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Sipeng]'s Articles
[Liu, Jifu]'s Articles
[Ding, Zhaoyang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Sipeng]'s Articles
[Liu, Jifu]'s Articles
[Ding, Zhaoyang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.