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Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch
Zhuang W.-J.3; Fong C.-C.3; Cao J.2; Ao L.2; Leung C.-H.3; Cheung H.-Y.3; Xiao P.-G.1; Fong W.-F.3; Yang M.-S.3
2004
Source PublicationPhytomedicine
ISSN09447113
Volume11Issue:4Pages:295-302
AbstractTripterygium hypoglaucum (levl.) Hutch (Celastraceae) (THH) root is a Chinese medicinal herb commonly used for treating autoimmune diseases. In the present study, alkaloids of THH were prepared and their cytotoxicity against the HL-60 cell was investigated. THH-induced apoptosis was observed using flow cytometry, confocal fluorescence microscope, and DNA laddering and caspase assays. The molecular mechanism involved in the induction of HL-60 cell apoptosis by THH alkaloids was examined using cDNA microarrays containing 3000 human genes derived from a leukocyte cDNA library. Sixteen genes were identified to be differentially expressed in HL-60 cells upon THH treatment. Several genes related to the NF-κB signaling pathway and cell apoptosis (such as NFKBIB, PRG1 and B2M) were up-regulated. In addition, c-myc binding protein and apoptosis-related cysteine proteases caspase-3 and caspase-8 were also regulated. The changes in c-Myc RNA expression and c-myc protein level were further confirmed by RT-PCR and Western blot analysis. The results demonstrated that THH alkaloids induced apoptosis of HL-60 cells though c-myc and NF-κB signaling pathways.
KeywordApoptosis CDNA microarray Cytotoxicity Tripterygium hypoglaucum (levl.) Hutch extracts
DOI10.1078/0944711041495128
URLView the original
Language英語
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Cited Times [WOS]:24   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Chinese Academy of Medical Sciences
2.Third Military Medical University
3.City University of Hong Kong
Recommended Citation
GB/T 7714
Zhuang W.-J.,Fong C.-C.,Cao J.,et al. Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch[J]. Phytomedicine,2004,11(4):295-302.
APA Zhuang W.-J..,Fong C.-C..,Cao J..,Ao L..,Leung C.-H..,...&Yang M.-S..(2004).Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch.Phytomedicine,11(4),295-302.
MLA Zhuang W.-J.,et al."Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (levl.) Hutch".Phytomedicine 11.4(2004):295-302.
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