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A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine
He H.-Z.1; Wang M.1; Chan D.S.H.1; Leung C.-H.2; Qiu J.-W.3; Ma D.-L.1
2013-12-15
Source PublicationMethods
ISSN10462023 10959130
Volume64Issue:3Pages:205-211
Abstract

A label-free G-quadruplex-based luminescent switch-on assay has been developed for the selective detection of micromolar histidine in aqueous solution. In this study, an iridium(III) complex was employed as a G-quadruplex-specific luminescent probe while a guanine-rich oligonucleotide (Pu27, 5′-TG4AG3TG4AG3TG4A2G2-3′)/cupric ion (Cu2+) ensemble was employed as a recognition unit for histidine. The initial luminescence of the iridium(III) complex in the presence of G-quadruplex DNA is effectively quenched by Cu2+ ions due to the Cu2+-mediated unfolding of the G-quadruplex motif. The addition of histidine sequesters Cu2+ ions from the ensemble, thereby restoring the luminescence of the system. The assay could detect down to 1 μM of histidine in aqueous media, and also exhibited good selectivity for histidine over other amino acids with the use of the cysteine, masking agent N-ethylmaleimide. Furthermore, the application of the assay for the detection of histidine in diluted urine samples was demonstrated.

KeywordCu2++ G-quadruplex Histidine Iridium(Iii) Complex Label-free Luminescence
DOI10.1016/j.ymeth.2013.07.023
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000328309600004
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Cited Times [WOS]:20   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Department of Biology, Hong Kong Baptist University, Hong Kong, China
Recommended Citation
GB/T 7714
He H.-Z.,Wang M.,Chan D.S.H.,et al. A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine[J]. Methods,2013,64(3):205-211.
APA He H.-Z.,Wang M.,Chan D.S.H.,Leung C.-H.,Qiu J.-W.,&Ma D.-L..(2013).A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine.Methods,64(3),205-211.
MLA He H.-Z.,et al."A label-free G-quadruplex-based luminescent switch-on assay for the selective detection of histidine".Methods 64.3(2013):205-211.
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