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A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity
Leung K.-H.1; He H.-Z.1; Zhong H.-J.2; Lu L.1; Chan D.S.H.1; Ma D.-L.1; Leung C.-H.2
2013-12-15
Source PublicationMethods
ISSN10462023 10959130
Volume64Issue:3Pages:224-228
Abstract

We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.

KeywordG-quadruplex Iridium(Iii) Complex Luminescent Polymerase Proofreading
DOI10.1016/j.ymeth.2013.05.017
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000328309600007
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.; Leung C.-H.
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Leung K.-H.,He H.-Z.,Zhong H.-J.,et al. A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity[J]. Methods,2013,64(3):224-228.
APA Leung K.-H..,He H.-Z..,Zhong H.-J..,Lu L..,Chan D.S.H..,...&Leung C.-H..(2013).A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity.Methods,64(3),224-228.
MLA Leung K.-H.,et al."A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity".Methods 64.3(2013):224-228.
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