UM  > 中華醫藥研究院
A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion
He H.-Z.1; Wang M.1; Chan D.S.H.1; Leung C.-H.2; Lin X.1,3,4; Lin J.-M.3; Ma D.-L.1
2013-12-15
Source PublicationMethods
ISSN10462023 10959130
Volume64Issue:3Pages:212-217
Abstract

A parallel G-quadruplex-selective iridium(III) complex has been synthesized and employed as a lumines-cent probe in a label-free G-quadruplex-based detection assay for Ca2+ions in aqueous solution. In thisassay, a guanine-rich oligonucleotide (G4, 50-G4T4G4-30) initially exists in an antiparallel G-quadruplexconformation, resulting in a low luminescence signal. Upon incubation with Ca2+ions, the antiparallelG-quadruplex is induced into a parallel G-quadruplex conformation, which greatly enhances the lumi-nescence emission of the iridium(III) probe. This method was highly sensitive for Ca2+ions with a limitof detection in the nanomolar range, and was selective for Ca2+over other metal ions.

KeywordCa2++ Ions G-quadruplex Iridium Luminescence Metal Complex
DOI10.1016/j.ymeth.2013.07.021
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS IDWOS:000328309600004
Fulltext Access
Citation statistics
Cited Times [WOS]:21   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China
4.State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
Recommended Citation
GB/T 7714
He H.-Z.,Wang M.,Chan D.S.H.,et al. A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion[J]. Methods,2013,64(3):212-217.
APA He H.-Z..,Wang M..,Chan D.S.H..,Leung C.-H..,Lin X..,...&Ma D.-L..(2013).A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion.Methods,64(3),212-217.
MLA He H.-Z.,et al."A parallel G-quadruplex-selective luminescent probe for the detection of nanomolar calcium(II) ion".Methods 64.3(2013):212-217.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[He H.-Z.]'s Articles
[Wang M.]'s Articles
[Chan D.S.H.]'s Articles
Baidu academic
Similar articles in Baidu academic
[He H.-Z.]'s Articles
[Wang M.]'s Articles
[Chan D.S.H.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[He H.-Z.]'s Articles
[Wang M.]'s Articles
[Chan D.S.H.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.