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Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex
Lin S.1; He B.1; Yang C.3; Leung C.-H.3; Mergny J.-L.2; Ma D.-L.1
2015
Source PublicationChemical Communications
ISSN1364548X 13597345
Volume51Issue:89Pages:16033-16036
Abstract

In this study, we synthesized a series of 9 luminescent iridium(iii) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(iii) complex 8 [Ir(pbtz)(dtbpy)]PF (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

DOI10.1039/c5cc06655g
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000363666000002
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Cited Times [WOS]:36   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hong Kong Baptist University
2.ARN : Regulations Naturelles et Artificielles
3.University of Macau
4.Institut Europeen de Chimie et Biologie
Recommended Citation
GB/T 7714
Lin S.,He B.,Yang C.,et al. Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex[J]. Chemical Communications,2015,51(89):16033-16036.
APA Lin S.,He B.,Yang C.,Leung C.-H.,Mergny J.-L.,&Ma D.-L..(2015).Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex.Chemical Communications,51(89),16033-16036.
MLA Lin S.,et al."Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex".Chemical Communications 51.89(2015):16033-16036.
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