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Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe
Wang M.2; He B.2; Lu L.2; Leung C.-H.3; Mergny J.-L.1; Ma D.-L.2
2015-08-05
Source PublicationBiosensors and Bioelectronics
ISSN18734235 09565663
Volume70Pages:338-344
AbstractWe have synthesized a series of luminescent iridium(III) complexes and investigated their ability to act as luminescent split G-quadruplex probes. After screening, the iridium(III) complex 1 [Ir(2-phenylquinoline)(3,4,7,8-tetramethyl-1,10-phenanthroline)]PF was validated as a highly-selective G-quadruplex probe and was utilized to construct a label-free intermolecular G-quadruplex-based assay for the selective and sensitive detection of LMP1 gene deletion. This "mix-and-detect" assay is simple and selective, and could detect down to 10nM of the target gene in aqueous solution with a linear range from 10 to 500nM. We also investigated the performance of our split G-quadruplex-based sensing platform for LMP1 gene deletion in the presence of cellular debris, demonstrating the robustness of this sensing system in biological samples. Comparative assays were also performed using either organic dyes or labeled oligonucleotides as signal-transducing agents.
KeywordG-quadruplex Gene deletion Iridium(III) complex Label-free detection Luminescent probe
DOI10.1016/j.bios.2015.03.047
URLView the original
Language英語
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.ARN : Regulations Naturelles et Artificielles
2.Hong Kong Baptist University
3.University of Macau
Recommended Citation
GB/T 7714
Wang M.,He B.,Lu L.,et al. Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe[J]. Biosensors and Bioelectronics,2015,70:338-344.
APA Wang M.,He B.,Lu L.,Leung C.-H.,Mergny J.-L.,&Ma D.-L..(2015).Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe.Biosensors and Bioelectronics,70,338-344.
MLA Wang M.,et al."Label-free luminescent detection of LMP1 gene deletion using an intermolecular G-quadruplex-based switch-on probe".Biosensors and Bioelectronics 70(2015):338-344.
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