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A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA
Wanhe Wang1; Tian-Shu Kang2; Philip Wai Hong Chan3,4; Jin-Jian Lu2; Xiu-Ping Chen2; Chung-Hang Leung2; Dik-Lung Ma1,5
2015-11-17
Source PublicationScience and Technology of Advanced Materials
ISSN14686996
Volume16Issue:6
Abstract

We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This 'mix-and-detect' methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+.

KeywordExonuclease Iii G-quadruplex Iridium(Iii) Mercury(Ii) Ion
DOI10.1088/1468-6996/16/6/065004
URLView the original
Indexed BySCI
Language英语
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Multidisciplinary
WOS IDWOS:000368333800004
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorChung-Hang Leung; Dik-Lung Ma
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.School of Chemistry, Monash University, Clayton, Victoria 3800, Australia
4.Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
5.Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wanhe Wang,Tian-Shu Kang,Philip Wai Hong Chan,et al. A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA[J]. Science and Technology of Advanced Materials,2015,16(6).
APA Wanhe Wang.,Tian-Shu Kang.,Philip Wai Hong Chan.,Jin-Jian Lu.,Xiu-Ping Chen.,...&Dik-Lung Ma.(2015).A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA.Science and Technology of Advanced Materials,16(6).
MLA Wanhe Wang,et al."A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA".Science and Technology of Advanced Materials 16.6(2015).
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