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Biofunctional Activities of Equisetum ramosissimum Extract: Protective Effects against Oxidation, Melanoma, and Melanogenesis
Pin-Hui Li1; Yu-Pin Chiu2; Chieh-Chih Shih3; Zhi-Hong Wen3; Laura Kaodichi Ibeto4,5; Shu-Hung Huang6,7,8,9; Chien Chih Chiu10; Dik-Lung Ma11; Chung-Hang Leung12; Yaw-Nan Chang2; Hui-Min David Wang1,3,7,13
2016
Source PublicationOxidative Medicine and Cellular Longevity
ISSN19420994 19420900
Volume2016
Abstract

Equisetum ramosissimum, a genus of Equisetaceae, is a medicinal plant that can be separated into ethyl acetate (EA), dichloromethane (DM), n-hexane (Hex), methanol (MeOH), and water extracts. EA extract was known to have potent antioxidative properties, reducing power, DPPH scavenging activity, and metal ion chelating activity. This study compared these five extracts in terms of their inhibiting effects on three human malignant melanomas: A375, A375.S2, and A2058. MTT assay presented the notion that both EA and DM extracts inhibited melanoma growth but did not affect the viabilities of normal dermal keratinocytes (HaCaT) or fibroblasts. Western blot analyses showed that both EA and DM extracts induced overexpression of caspase proteins in all three melanomas. To determine their roles in melanogenesis, this study analyzed their in vitro suppressive effects on mushroom tyrosinase. All extracts except for water revealed moderate suppressive effects. None of the extracts affected B16-F10 cells proliferation. EA extract inhibited cellular melanin production whereas DM extract unexpectedly enhanced cellular pigmentation in B16-F10 cells. Data for modulations of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1, and tyrosinase-related protein 2 showed that EA extract inhibited protein expression mentioned above whereas DM extract had the opposite effect. Overall, the experiments indicated that the biofunctional activities of EA extract contained in food and cosmetics protect against oxidation, melanoma, and melanin production.

DOI10.1155/2016/2853543
URLView the original
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000378762100001
Fulltext Access
Citation statistics
Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, San Ming District, Kaohsiung 807, Taiwan
2.Department of Biotechnology, National Formosa University, 64 Wenhua Road, Huwei Township, Yunlin County 632, Taiwan
3.Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, 70 Lianhai Road, Gushan District, Kaohsiung 804, Taiwan
4.Department of Biological Science, University of North Carolina at Chapel Hill, Coker Hall, CB No. 3280, 120 South Road, Chapel Hill, NC 27599-3280, USA
5.Department of Biomedical Science, Barry University, 11300 Northeast 2nd Avenue, Miami, FL 33161, USA
6.Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, San Ming District, Kaohsiung 807, Taiwan
7.Center for Stem Cell Research, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, San Ming District, Kaohsiung 807, Taiwan
8.Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
9.Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
10.Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
11.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong
12.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau
13.Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Recommended Citation
GB/T 7714
Pin-Hui Li,Yu-Pin Chiu,Chieh-Chih Shih,et al. Biofunctional Activities of Equisetum ramosissimum Extract: Protective Effects against Oxidation, Melanoma, and Melanogenesis[J]. Oxidative Medicine and Cellular Longevity,2016,2016.
APA Pin-Hui Li.,Yu-Pin Chiu.,Chieh-Chih Shih.,Zhi-Hong Wen.,Laura Kaodichi Ibeto.,...&Hui-Min David Wang.(2016).Biofunctional Activities of Equisetum ramosissimum Extract: Protective Effects against Oxidation, Melanoma, and Melanogenesis.Oxidative Medicine and Cellular Longevity,2016.
MLA Pin-Hui Li,et al."Biofunctional Activities of Equisetum ramosissimum Extract: Protective Effects against Oxidation, Melanoma, and Melanogenesis".Oxidative Medicine and Cellular Longevity 2016(2016).
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