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A luminescent G-quadruplex-selective iridium(iii) complex for the label-free detection of lysozyme
Lihua Lu1; Wanhe Wang1; Modi Wang1; Tian-Shu Kang2; Jin-Jian Lu2; Xiu-Ping Chen2; Quan-Bin Han3; Chung-Hang Leung2; Dik-Lung Ma1
2016-04-14
Source PublicationJournal of Materials Chemistry B
ISSN2050750X 20507518
Volume4Issue:14Pages:2407-2411
Abstract

A novel Ir(iii) complex 1 displays high selectivity for the G-quadruplex, and was used to establish a label-free G-quadruplex-based detection platform for lysozyme in buffer. In this study, we employed a special feature of most G-quadruplex probes that they do not respond towards the TBA G-quadruplex. A duplex DNA with a TBA G-quadruplex tail was designed for the detection of lysozyme. The presence of lysozyme will induce duplex dissociation and release the hybridized c-kit87 G-quadruplex, which would be recognized by the Ir(iii) complex to generate a strong luminescence response. Common duplex DNA designs lacking a TBA G-quadruplex tail typically contain long complementary DNA regions that may be too stable to be dissociated, thus decreasing sensitivity. We anticipate that the presence of a TBA tail in the DNA duplex may destabilize the duplex structure, allowing the aptamer to be more easily dissociated and bind to the lysozyme, thereby increasing the sensitivity of the lysozyme detection platform. To our knowledge, this is the first example of the use of the TBA G-quadruplex to improve sensitivity through fine-tuning duplex stability. We believe that this approach may be further employed in sensing platforms for other targets. This assay exhibited a linear response for lysozyme within the concentration range of 2-50 nM (R = 0.9904), and the limit of detection for lysozyme was 2 nM. Moreover, this platform exhibited a potential use for biological sample analysis.

DOI10.1039/c6tb00426a
URLView the original
Indexed BySCI
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, bioMaterials
WOS IDWOS:000373442600002
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Cited Times [WOS]:20   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorDik-Lung Ma
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowloon Tong, Hong Kong, China
Recommended Citation
GB/T 7714
Lihua Lu,Wanhe Wang,Modi Wang,et al. A luminescent G-quadruplex-selective iridium(iii) complex for the label-free detection of lysozyme[J]. Journal of Materials Chemistry B,2016,4(14):2407-2411.
APA Lihua Lu.,Wanhe Wang.,Modi Wang.,Tian-Shu Kang.,Jin-Jian Lu.,...&Dik-Lung Ma.(2016).A luminescent G-quadruplex-selective iridium(iii) complex for the label-free detection of lysozyme.Journal of Materials Chemistry B,4(14),2407-2411.
MLA Lihua Lu,et al."A luminescent G-quadruplex-selective iridium(iii) complex for the label-free detection of lysozyme".Journal of Materials Chemistry B 4.14(2016):2407-2411.
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