UM
Chemical structure and immunomodulating activities of an α-glucan purified from Lobelia chinensis Lour
Li X.-J.3; Bao W.-R.1; Leung C.-H.2; Ma D.-L.1; Zhang G.1; Lu A.-P.1; Wang S.-C.3; Han Q.-B.1
2016-06-01
Source PublicationMolecules
ISSN14203049
Volume21Issue:6
AbstractA neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →α-D-Glcp→α-D-Glcp→(α-D-Glcp)x-α-D-Glcp-(α-D-Glcp)y→. The side chains were terminal α-D-Glcp1→ and α-D-Glcp1→ (α-D-Glcp)z→α-D-Glcp→α-D-Glcp→α-D-Glcp→ (x + y + z = 5), which are attached to the backbone at O-3 of α-D-Glcp. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects.
KeywordImmunomodulating Lobelia chinensis NMR RAW 264.7 TLR4
DOI10.3390/molecules21060779
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Hong Kong Baptist University
2.University of Macau
3.Shanghai University of Traditional Chinese Medicine
Recommended Citation
GB/T 7714
Li X.-J.,Bao W.-R.,Leung C.-H.,et al. Chemical structure and immunomodulating activities of an α-glucan purified from Lobelia chinensis Lour[J]. Molecules,2016,21(6).
APA Li X.-J..,Bao W.-R..,Leung C.-H..,Ma D.-L..,Zhang G..,...&Han Q.-B..(2016).Chemical structure and immunomodulating activities of an α-glucan purified from Lobelia chinensis Lour.Molecules,21(6).
MLA Li X.-J.,et al."Chemical structure and immunomodulating activities of an α-glucan purified from Lobelia chinensis Lour".Molecules 21.6(2016).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li X.-J.]'s Articles
[Bao W.-R.]'s Articles
[Leung C.-H.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li X.-J.]'s Articles
[Bao W.-R.]'s Articles
[Leung C.-H.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li X.-J.]'s Articles
[Bao W.-R.]'s Articles
[Leung C.-H.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.