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Comprehensive quantitative analysis of Chinese patent drug YinHuang drop pill by ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry
Wong T.-L.1; An Y.-Q.4; Yan B.-C.4; Yue R.-Q.1; Zhang T.-B.1; Ho H.-M.1; Ren T.-J.1; Fung H.-Y.1; Ma D.-L.1; Leung C.-H.3; Liu Z.-L.2; Pu J.-X.4; Han Q.-B.1; Sun H.-D.4
2016-06-05
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN1873264X 07317085
Volume125Pages:415-426
AbstractYinHuang drop pill (YHDP) is a new preparation, derived from the traditional YinHuang (YH) decoction. Since drop pills are one of the newly developed forms of Chinese patent drugs, not much research has been done regarding the quality and efficacy. This study aims to establish a comprehensive quantitative analysis of the chemical profile of YHDP. ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to identify 34 non-sugar small molecules including 15 flavonoids, 9 phenolic acids, 5 saponins, 1 iridoid, and 4 iridoid glycosides in YHDP samples, and 26 of them were quantitatively determined. Sugar composition of YHDP in terms of fructose, glucose and sucrose was examined via a high performance liquid chromatography-evaporative light scattering detector on an amide column (HPLC-NHP-ELSD). Macromolecules were examined by high performance gel permeation chromatography coupled with ELSD (HPGPC-ELSD). The content of the drop pill's skeleton component PEG-4000 was also quantified via ultra-high performance liquid chromatography coupled with charged aerosol detector (UHPLC-CAD). The results showed that up to 73% (w/w) of YHDP could be quantitatively determined. Small molecules accounted for approximately 5%, PEG-4000 represented 68%, while no sugars or macromolecules were found. Furthermore, YHDP showed no significant differences in terms of daily dosage, compared to YinHuang granules and YinHuang oral liquid; however, it has a higher small molecules content compared to YinHuang lozenge.
KeywordChinese medicine formulas Quality evaluation Quantitative analysis YinHuang drop pill
DOI10.1016/j.jpba.2016.04.008
URLView the original
Language英語
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Hong Kong Baptist University
2.Zhejiang WeiKang Pharmaceutical Co., Ltd
3.University of Macau
4.Kunming Institute of Botany Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wong T.-L.,An Y.-Q.,Yan B.-C.,et al. Comprehensive quantitative analysis of Chinese patent drug YinHuang drop pill by ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis,2016,125:415-426.
APA Wong T.-L..,An Y.-Q..,Yan B.-C..,Yue R.-Q..,Zhang T.-B..,...&Sun H.-D..(2016).Comprehensive quantitative analysis of Chinese patent drug YinHuang drop pill by ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry.Journal of Pharmaceutical and Biomedical Analysis,125,415-426.
MLA Wong T.-L.,et al."Comprehensive quantitative analysis of Chinese patent drug YinHuang drop pill by ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry".Journal of Pharmaceutical and Biomedical Analysis 125(2016):415-426.
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