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Interaction of an Iridium(III) Complex with G-Quadruplex DNA and Its Application in Luminescent Switch-On Detection of Siglec-5
Lin S.2; Lu L.2; Kang T.-S.4; Mergny J.-L.5; Leung C.-H.4; Ma D.-L.2
2016-10-18
Source PublicationAnalytical Chemistry
ISSN15206882 00032700
Volume88Issue:20Pages:10290-10295
Abstract

Sialic acid (Sia) binding immunoglobulin (Ig)-like lectin-5 (Siglec-5) is a type-I transmembrane protein, and it has been demonstrated as a biomarker of granulocytic maturation and acute myeloid leukemia phenotype. Herein we aimed to construct a method that could sensitively detect Siglec-5 by taking advantage of the high affinity and selectivity of the K19 aptamer for its cognate target, and the selective interaction of luminescent iridium(III) transition metal complexes with G-quadruplex DNA. The iridium(III) complex 1 [Ir(tpyd)(2,9-dmphen)]PF (where tpyd =2-(m-tolyl)pyridine; 2,9-dmphen =2,9-dimethyl-1,10-phenanthroline) was synthesized, and it displayed high luminescence for G-quadruplex DNA compared to dsDNA and ssDNA. Additionally, complex 1 exhibited a blue shift luminescence response to c-kit2 G-quadruplex, and the interaction between 1 and G-quadruplexes was discussed based on the results of G-tetrad assay, loop effect assay, and other assays. Then complex 1 was utilized to develop a G-quadruplex-based sensing platform for Siglec-5 in aqueous solution. Upon the addition of Siglec-5, the specific binding of the K19 aptamer sequence results in a conformational change that generates a split G-quadruplex structure, which is then recognized by the G-quadruplex-specific iridium(III) complex with an enhanced luminescent response. Futhermore, the use of the assay for detecting Siglec-5 in cellular debris was demonstrated.

DOI10.1021/acs.analchem.6b03128
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000385907400057
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Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.ARN : Regulations Naturelles et Artificielles
2.Hong Kong Baptist University
3.Qingdao Agricultural University
4.University of Macau
5.Institut Europeen de Chimie et Biologie
Recommended Citation
GB/T 7714
Lin S.,Lu L.,Kang T.-S.,et al. Interaction of an Iridium(III) Complex with G-Quadruplex DNA and Its Application in Luminescent Switch-On Detection of Siglec-5[J]. Analytical Chemistry,2016,88(20):10290-10295.
APA Lin S.,Lu L.,Kang T.-S.,Mergny J.-L.,Leung C.-H.,&Ma D.-L..(2016).Interaction of an Iridium(III) Complex with G-Quadruplex DNA and Its Application in Luminescent Switch-On Detection of Siglec-5.Analytical Chemistry,88(20),10290-10295.
MLA Lin S.,et al."Interaction of an Iridium(III) Complex with G-Quadruplex DNA and Its Application in Luminescent Switch-On Detection of Siglec-5".Analytical Chemistry 88.20(2016):10290-10295.
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