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A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex
Lu L.2; Mao Z.1; Kang T.-S.3; Leung C.-H.3; Ma D.-L.1
2016-11-15
Source PublicationBiosensors and Bioelectronics
ISSN18734235 09565663
Volume85Pages:300-309
Abstract

The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the development of a versatile nanomachine. In the nanomachine, the single-stranded DNA (ssDNA) oligonucleotide ON1 hybridizes with the hairpin DNA oligonucleotide ON2, leading to the formation of a 5'-recessed double-stranded DNA (dsDNA) structure and the simultaneous release of a G-rich DNA sequence, which folds into a G-quadruplex motif that is recognized by complex 1. Upon the addition of Exo III, the dsDNA substrate is digested leading to the liberation of ON1 ssDNA, which enters a new cycle of the nanomachine. This nanomachine was successfully used to detect PDGF-BB by combination with a simple pre-procedure module, and exhibited a linear response between luminescence intensity and PDGF-BB concentration in the range of 10 to 300 pM (R = 0.992), with a limit of detection for PDGF-BB of 10 pM. This assay was highly selective for PDGF-BB over other proteins, and exhibited potential use in biological sample analysis. Moreover, the versatility of the nanomachine was demonstrated by adapting the nanomachine for the detection of thrombin by simply changing the pre-procedure module.

KeywordG-quadruplex Iridium(Iii) Complex Luminescent Nanomachine Pdgf-bb
DOI10.1016/j.bios.2016.05.026
URLView the original
Indexed BySCI
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000382410100040
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Cited Times [WOS]:25   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hong Kong Baptist University
2.Qingdao Agricultural University
3.University of Macau
Recommended Citation
GB/T 7714
Lu L.,Mao Z.,Kang T.-S.,et al. A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex[J]. Biosensors and Bioelectronics,2016,85:300-309.
APA Lu L.,Mao Z.,Kang T.-S.,Leung C.-H.,&Ma D.-L..(2016).A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex.Biosensors and Bioelectronics,85,300-309.
MLA Lu L.,et al."A versatile nanomachine for the sensitive detection of platelet-derived growth factor-BB utilizing a G-quadruplex-selective iridium(III) complex".Biosensors and Bioelectronics 85(2016):300-309.
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