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A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity
Lin S.1; Kang T.-S.3; Lu L.1; Wang W.1; Ma D.-L.1; Leung C.-H.3
2016-12-15
Source PublicationBiosensors and Bioelectronics
ISSN18734235 09565663
Volume86Pages:849-857
Abstract

Thymine DNA glycosylase (TDG) performs essential functions in maintaining genetic integrity and epigenetic regulation, which also plays an essential role in DNA demethylation. In this work, the novel iridium(III) complex 1 with an anchor tail was synthesized and employed to construct a G-quadruplex-based assay for detecting TDG activity in aqueous solution by using the mismatched base excising property of TDG with T4 DNA ligase and phi29 DNA polymerase, in concert with the rolling circle amplification (RCA) strategy. The assay achieved a detection limit of 0.048 U mL (0.012 ng mL), and showed high selectivity towards TDG even in the presence of other proteins and enzymes. Additionally, the assay could function in diluted cellular debris.

KeywordG-quadruplex Iridium(Iii) Complex Luminescence Tdg
DOI10.1016/j.bios.2016.07.082
URLView the original
Indexed BySCI
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000384853300116
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Cited Times [WOS]:22   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hong Kong Baptist University
2.Qingdao Agricultural University
3.University of Macau
Recommended Citation
GB/T 7714
Lin S.,Kang T.-S.,Lu L.,et al. A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity[J]. Biosensors and Bioelectronics,2016,86:849-857.
APA Lin S.,Kang T.-S.,Lu L.,Wang W.,Ma D.-L.,&Leung C.-H..(2016).A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity.Biosensors and Bioelectronics,86,849-857.
MLA Lin S.,et al."A G-quadruplex-selective luminescent probe with an anchor tail for the switch-on detection of thymine DNA glycosylase activity".Biosensors and Bioelectronics 86(2016):849-857.
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