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First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells
Wang W.2; Yang C.4; Lin S.2; Vellaisamy K.2; Li G.4; Tan W.3; Leung C.-H.4; Ma D.-L.2
2017-04-06
Source PublicationChemistry - A European Journal
ISSN15213765 09476539
Volume23Issue:20Pages:4929-4935
Abstract

NF-κB is a critical transcription factor that plays an important role in mediating inflammation, the immune response, and cell proliferation. The activation of NF-κB leads to an enhancement of proinflammatory mediator expression, which is implicated in the pathogenesis of a variety of diseases. Therefore, methods that allow the intracellular tracking of NF-κB are particularly attractive because they can provide information regarding the pathways or stimulation responses that are involved in the activation of NF-κB. In this work, we report a novel platform to track intracellular NF-κB by employing the conjugated iridium(III) complex 1, which was synthesized through the unique combination of a luminescent iridium(III) moiety with the natural product oridonin. Experiments conducted with p50 knockdown cells revealed that complex 1 could detect the p50 subunit of NF-κB in cellulo. Furthermore, complex 1 tracked NF-κB translocation induced by tumor necrosis factor-α (TNF-α) as a model stimulus, without affecting the translocation process itself. To the best of our knowledge, complex 1 is the first metal-based compound that has been reported to be capable of monitoring intracellular NF-κB in living cells.

KeywordCell Imaging Iridium(Iii) Complex Natural Products Nf-κb
DOI10.1002/chem.201700770
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000399327100026
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Hunan University
2.Hong Kong Baptist University
3.University of Florida
4.University of Macau
Recommended Citation
GB/T 7714
Wang W.,Yang C.,Lin S.,et al. First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells[J]. Chemistry - A European Journal,2017,23(20):4929-4935.
APA Wang W..,Yang C..,Lin S..,Vellaisamy K..,Li G..,...&Ma D.-L..(2017).First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells.Chemistry - A European Journal,23(20),4929-4935.
MLA Wang W.,et al."First Synthesis of an Oridonin-Conjugated Iridium(III) Complex for the Intracellular Tracking of NF-κB in Living Cells".Chemistry - A European Journal 23.20(2017):4929-4935.
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