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Silencing stem cell factor gene in fibroblasts to regulate paracrine factor productions and enhance c-Kit expression in melanocytes on melanogenesis
Li P.-H.1; Liu L.-H.2; Chang C.-C.2; Gao R.5; Leung C.-H.4; Ma D.-L.3; David Wang H.-M.2
2018-05-16
Source PublicationInternational Journal of Molecular Sciences
ISSN14220067 16616596
Volume19Issue:5
Abstract

Melanogenesis is a complex physiological mechanism involving various paracrine factors. Skin cells such as keratinocytes, fibroblasts, and melanocytes communicate with one another through secreted regulators, thereby regulating the melanocytes’ bio-functions. The stem cell factor (SCF) is a paracrine factor produced by fibroblasts, and its receptor, c-kit, is expressed on melanocytes. Binding of SCF to c-kit activates autophosphorylation and tyrosine kinase to switch on its signal transmission. SCF inhibition does not suppress fibroblast proliferation in MTT assay, and SCF silencing induced mRNA expressions of paracrine factor genes, HGF, NRG-1, and CRH in qPCR results. Following UVB stimulation, gene expressions of HGF, NRG, and CRH were higher than homeostasis; in particular, HGF exhibited the highest correlation with SCF variations. We detected fibroblasts regulated SCF in an autocrine-dependent manner, and the conditioned medium obtained from fibroblast culture was applied to treat melanocytes. Melanogenesis-related genes, tyrosinase and pmel17, were upregulated under conditioned mediums with SCF silencing and exposed to UVB treatments. Melanin quantities in the melanocytes had clearly increased in the pigment content assay. In conclusion, SCF silencing causes variations in both fibroblast paracrine factors and melanocyte melanogenesis, and the differences in gene expressions were observed following UVB exposure.

KeywordFibroblasts Melanin Melanocytes Paracrine Factors Stem Cell Factor
DOI10.3390/ijms19051475
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000435297000211
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Kaohsiung Medical University
2.National Chung Hsing University
3.Hong Kong Baptist University
4.University of Macau
5.Tsinghua University
6.Quanzhou Normal University
Recommended Citation
GB/T 7714
Li P.-H.,Liu L.-H.,Chang C.-C.,et al. Silencing stem cell factor gene in fibroblasts to regulate paracrine factor productions and enhance c-Kit expression in melanocytes on melanogenesis[J]. International Journal of Molecular Sciences,2018,19(5).
APA Li P.-H..,Liu L.-H..,Chang C.-C..,Gao R..,Leung C.-H..,...&David Wang H.-M..(2018).Silencing stem cell factor gene in fibroblasts to regulate paracrine factor productions and enhance c-Kit expression in melanocytes on melanogenesis.International Journal of Molecular Sciences,19(5).
MLA Li P.-H.,et al."Silencing stem cell factor gene in fibroblasts to regulate paracrine factor productions and enhance c-Kit expression in melanocytes on melanogenesis".International Journal of Molecular Sciences 19.5(2018).
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