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Simultaneous quantification of major flavonoids in "bawanghua", the edible flower of Hylocereus undatus using pressurised liquid extraction and high performance liquid chromatography
Yi Y.1; Zhang Q.-W.1; Li S.-L.1; Wang Y.3; Ye W.-C.3; Zhao J.1; Wang Y.-T.1
2012-11-15
Source PublicationFood Chemistry
ISSN03088146 18737072
Volume135Issue:2Pages:528-533
Abstract

A pressurised liquid extraction (PLE) and high performance liquid chromatography (HPLC) method was developed for simultaneous quantification of six major flavonoids in edible flower of Hylocereus undatus. In order to achieve the baseline separation of two pairs of isomers, the HPLC conditions were optimised with different kind of reversed phase columns and mobile phase gradient programs. In addition, the solvent concentration, extraction temperature, extraction time and flush cycle for PLE were also optimised. Zorbax SB-C8 (100 × 2.1 mm, 1.8 μm) column was chosen with acetonitrile and water containing 0.1% trifluoroacetic acid as mobile phase, the six analytes were eluted with baseline separation. The calibration curves showed good linearity (r > 0.9994) with LODs and LOQs less than 0.90 and 3.60 ng respectively. The RSDs for intra- and inter-day repeatability was not more than 1.09% and 1.79% respectively. The overall recovery of the assay was 96.9-105.2%. The sample was stable for at least 12 h. The newly established method was successfully applied to quantify six flavonoids in different parts of "Bawanghua", and the commercial samples from different locations. © 2012 Elsevier Ltd. All rights reserved.

KeywordBawanghua Flavonoid Hplc Hylocereus Undatus Ple
DOI10.1016/j.foodchem.2012.05.010
URLView the original
Indexed BySCI
WOS Research AreaChemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS SubjectChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000308574300032
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Cited Times [WOS]:20   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.University of Macau
2.Jiangsu Province Academy of Chinese Medicine
3.Jinan University
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Yi Y.,Zhang Q.-W.,Li S.-L.,et al. Simultaneous quantification of major flavonoids in "bawanghua", the edible flower of Hylocereus undatus using pressurised liquid extraction and high performance liquid chromatography[J]. Food Chemistry,2012,135(2):528-533.
APA Yi Y..,Zhang Q.-W..,Li S.-L..,Wang Y..,Ye W.-C..,...&Wang Y.-T..(2012).Simultaneous quantification of major flavonoids in "bawanghua", the edible flower of Hylocereus undatus using pressurised liquid extraction and high performance liquid chromatography.Food Chemistry,135(2),528-533.
MLA Yi Y.,et al."Simultaneous quantification of major flavonoids in "bawanghua", the edible flower of Hylocereus undatus using pressurised liquid extraction and high performance liquid chromatography".Food Chemistry 135.2(2012):528-533.
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