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Qualitative and quantitative analysis of volatile components in Pogostemon Cablin by head-space solid phase microextraction and gas chromatography-mass spectrometry
Lian Z.-Y.; Yang F.-Q.; Li S.-P.
2009
Source PublicationFenxi Huaxue/ Chinese Journal of Analytical Chemistry
ISSN18722040 2533820
Volume37Issue:2Pages:283-287
AbstractA head-space solid phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of volatile components in dry Pogostemon. Based on the peak area of patchouli alcohol, the experimental conditions for HS-SPME were optimized. The optimum conditions were; 30 mg Pogostemon cablin powder, which was 10 times diluted using anhydrous sodium sulfate, was placed into a 15 mL extraction tube. After incubated at 80 °C for 40 min with stirring speed of 250 r/min, polydimethylsiloxane/divinylbenzene(PDMS-DVB) coated fiber was inserted and extracted for 40 min at 80 °C with the same stirring speed. Then the fiber was inserted into the injection port and desorption for 100 s at 250 °C. DB-5MS capillary with a flow rate of 1 mL/min was used for GC separation. The average recovery of patchouli alcohol under the optimum conditions was 91. 8% with a RSD of 3. 0%. Then the content of patchouli alcohol in ten samples of Pogostemon cablin from different areas was determined, the other main volatile components in Pogostemon were also calculated by using patchouli alcohol as reference standard. The results show that the developed method is simple, rapid and reliable, which is suitable for qualitative and quantitative analysis of volatile compounds in Pogostemon cablin.
KeywordGas chromatography-mass spectrometry Head-space solid phase microextraction Patchouli alcohol Pogostemon
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversity of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Lian Z.-Y.,Yang F.-Q.,Li S.-P.. Qualitative and quantitative analysis of volatile components in Pogostemon Cablin by head-space solid phase microextraction and gas chromatography-mass spectrometry[J]. Fenxi Huaxue/ Chinese Journal of Analytical Chemistry,2009,37(2):283-287.
APA Lian Z.-Y.,Yang F.-Q.,&Li S.-P..(2009).Qualitative and quantitative analysis of volatile components in Pogostemon Cablin by head-space solid phase microextraction and gas chromatography-mass spectrometry.Fenxi Huaxue/ Chinese Journal of Analytical Chemistry,37(2),283-287.
MLA Lian Z.-Y.,et al."Qualitative and quantitative analysis of volatile components in Pogostemon Cablin by head-space solid phase microextraction and gas chromatography-mass spectrometry".Fenxi Huaxue/ Chinese Journal of Analytical Chemistry 37.2(2009):283-287.
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