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Use of HPTLC to differentiate among the crude polysaccharides in six traditional Chinese medicines
Yang C.; Guan J.; Zhang J.-S.; Li S.-P.
2010-02-01
Source PublicationJournal of Planar Chromatography - Modern TLC
ISSN09334173
Volume23Issue:1Pages:46-49
AbstractA simple, rapid, and effective high-performance thin-layer chromatographic (HPTLC) method has been established for differentiating among the polysaccharides present in six traditional Chinese medicines (TCM), Cordyceps sinensis, Ganoderma lucidum, Astragalus memberanaceus, Panax ginseng, Panax quinquefolii, and Panax notogiseng. Acid hydrolyzates of the polysaccharides were analyzed by HPTLC with two detection reagents, aniline-diphenylamine- phosphoric acid and ninhydrin, and scanning densitometry. The compounds were separated on silica gel plates with chloroform-n-butanol-methanol-acetic acid-water 4.5:12.5:5:1.5:1.5 (v/v) as mobile phase. Seven monosaccharides and two glucuronic acids were used as reference compounds. The results showed that hydrolysis of polysaccharides can release specific molecules present in the herbal species in addition to the monosaccharides present. This is useful for distinguishing the origins of the polysaccharides in Chinese medicines. © Akadémiai Kiadó, Budapest.
KeywordHPTLC Polysaccharides Quality control Traditional Chinese medicines (TCM)
DOI10.1556/JPC.23.2010.1.8
URLView the original
Language英語
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversity of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Yang C.,Guan J.,Zhang J.-S.,et al. Use of HPTLC to differentiate among the crude polysaccharides in six traditional Chinese medicines[J]. Journal of Planar Chromatography - Modern TLC,2010,23(1):46-49.
APA Yang C.,Guan J.,Zhang J.-S.,&Li S.-P..(2010).Use of HPTLC to differentiate among the crude polysaccharides in six traditional Chinese medicines.Journal of Planar Chromatography - Modern TLC,23(1),46-49.
MLA Yang C.,et al."Use of HPTLC to differentiate among the crude polysaccharides in six traditional Chinese medicines".Journal of Planar Chromatography - Modern TLC 23.1(2010):46-49.
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