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Analysis of global components in Ganoderma using liquid chromatography system with multiple columns and detectors
Qian Z.; Zhao J.; Li D.; Hu D.; Li S.
2012-10-01
Source PublicationJournal of Separation Science
ISSN16159306 16159314
Volume35Issue:20Pages:2725-2734
Abstract

In present study, a multiple columns and detectors liquid chromatography system for analysis of global components in traditional Chinese medicines was developed. The liquid chromatography system was consist of three columns, including size exclusion chromatography column, hydrophilic interaction chromatography column, and reversed phase chromato-graphy column, and three detectors, such as diode array detector, evaporative light scattering detector, and mass spectrometry detector, based on column switching technique. The developed multiple columns and detectors liquid chromatography system was successfully applied to the analysis of global components, including macromolecular (polysaccharides), high (nucleosides and sugars)-, and low (triterpenes)-polarity small molecular compounds in Ganoderma, a well-known Chinese medicinal mushroom. As a result, one macromolecular chromatographic peak was found in two Ganoderma species, 19 components were identified in Ganoderma lucidum (two sugars, three nucleosides, and 14 triterpenes), and four components (two sugars and two nucleosides) were identified in Ganoderma sinense. The developed multiple columns and detectors liquid chromatography system was helpful to understand comprehensive chemical characters in TCMs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KeywordGanoderma Lucidum Ganoderma Sinense Liquid Chromatography Multiple Columns Multiple Detectors
DOI10.1002/jssc.201200441
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000309916500008
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversity of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Qian Z.,Zhao J.,Li D.,et al. Analysis of global components in Ganoderma using liquid chromatography system with multiple columns and detectors[J]. Journal of Separation Science,2012,35(20):2725-2734.
APA Qian Z.,Zhao J.,Li D.,Hu D.,&Li S..(2012).Analysis of global components in Ganoderma using liquid chromatography system with multiple columns and detectors.Journal of Separation Science,35(20),2725-2734.
MLA Qian Z.,et al."Analysis of global components in Ganoderma using liquid chromatography system with multiple columns and detectors".Journal of Separation Science 35.20(2012):2725-2734.
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