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Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis using high performance liquid chromatography coupled with diode array and evaporative light scattering detection
Peng B.2; Qiao C.-F.2; Zhao J.2; Huang W.-H.2; Hu D.-J.2; Liu H.-G.1; Li S.-P.2
2013-03-01
Source PublicationMolecules
ISSN14203049
Volume18Issue:3Pages:2934-2941
Abstract

A high performance liquid chromatography coupled with diode array and evaporative light scattering detection (HPLC-DAD-ELSD) method for simultaneous determination of eight major bioactive compounds including two flavonoids (rutin and eriodictyol-7-O-β-D-glucopyranoside), two isochlorogenic acids (isochlorogenic acid A and isochlorogenic acid C) and four triterpenoids (ilexhainanoside D, ilexsaponin A1, ilexgenin A and ursolic acid) in Ilex hainanensis has been developed for the first time. The 283 nm wavelength was chosen for determination of two flavonoids and two isochlorogenic acids. ELSD was applied to determine four triterpenoids. The analysis was performed on an Agilent Zorbax SB-C column (250 × 4.6 mm i.d., 5 μm) with gradient elution of 0.2% formic acid in water and acetonitrile. The method was validated for linearity, limit of detection, limit of quantification, precision, repeatability and accuracy. The proposed method has been successfully applied for simultaneous quantification of the analytes in four samples of Ilex hainanensis, which is helpful for quality control of this plant.

KeywordFlavonoid Hplc-dad-elsd Ilex Hainanensis Isochlorogenic Acid Triterpenoid
DOI10.3390/molecules18032934
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000316611700036
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Guangxi Medical University
2.University of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Peng B.,Qiao C.-F.,Zhao J.,et al. Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis using high performance liquid chromatography coupled with diode array and evaporative light scattering detection[J]. Molecules,2013,18(3):2934-2941.
APA Peng B..,Qiao C.-F..,Zhao J..,Huang W.-H..,Hu D.-J..,...&Li S.-P..(2013).Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis using high performance liquid chromatography coupled with diode array and evaporative light scattering detection.Molecules,18(3),2934-2941.
MLA Peng B.,et al."Simultaneous determination of flavonoids, isochlorogenic acids and triterpenoids in Ilex hainanensis using high performance liquid chromatography coupled with diode array and evaporative light scattering detection".Molecules 18.3(2013):2934-2941.
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