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Determination of fructooligosaccharides in burdock using HPLC and microwave-assisted extraction
Li J.2; Liu X.2; Zhou B.2; Zhao J.2; Li S.2
2013-06-19
Source PublicationJournal of Agricultural and Food Chemistry
ISSN00218561 15205118
Volume61Issue:24Pages:5888-5892
Abstract

The root of burdock (Arctium lappa L.) is a commonly used vegetable in Asia. Fructooligosaccharides (FOS) are usually considered as its main bioactive components. Thus, quantitative analysis of these components is very important for the quality control of burdock. In this study, an HPLC-ELSD and microwave-assisted extraction method was developed for the simultaneous determination of seven FOS with degrees of polymerization (DP) between 3 and 9, as well as fructose, glucose, and sucrose in burdock from different regions. The separation was performed on a Waters XBridge Amide column (4.6 × 250 mm i.d., 3.5 μm) with gradient elution. All calibration curves for investigated analytes showed good linear regression (r > 0.9990). Their LODs and LOQs were lower than 3.63 and 24.82 μg/mL, respectively. The recoveries ranged from 99.2 to 102.6%. The developed method was successfully applied to determination of ten sugars in burdock from different locations of Asia. The results showed that the contents of FOS in different samples of burdock collected at appropriate times were similar, and the developed HPLC-ELSD with microwave-assisted extraction method is helpful to control the quality of burdock. © 2013 American Chemical Society.

KeywordBurdock Carbohydrates Fructooligosaccharide Hplc-elsd Microwave-assisted Extraction
DOI10.1021/jf400534n
URLView the original
Indexed BySCI
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000320898600033
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Cited Times [WOS]:21   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Jiangxi Science and Technology Normal University
2.University of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Li J.,Liu X.,Zhou B.,et al. Determination of fructooligosaccharides in burdock using HPLC and microwave-assisted extraction[J]. Journal of Agricultural and Food Chemistry,2013,61(24):5888-5892.
APA Li J.,Liu X.,Zhou B.,Zhao J.,&Li S..(2013).Determination of fructooligosaccharides in burdock using HPLC and microwave-assisted extraction.Journal of Agricultural and Food Chemistry,61(24),5888-5892.
MLA Li J.,et al."Determination of fructooligosaccharides in burdock using HPLC and microwave-assisted extraction".Journal of Agricultural and Food Chemistry 61.24(2013):5888-5892.
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