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Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection
Li J.2; Cheong K.L.2; Zhao J.2; Hu D.J.2; Chen X.Q.2; Qiao C.F.2; Zhang Q.W.2; Chen Y.W.2; Li S.P.2
2013-09-20
Source PublicationJournal of Chromatography A
ISSN00219673 18733778
Volume1308Pages:52-57
Abstract

A fast protein liquid chromatography coupled with refractive index detection (FPLC-RID) method was firstly developed for preparation and purification of fructooligosaccharides with different degree of polymerization from burdock, Arctium lappa. After extraction with 60% ethanol and decolorization with MCI gel CHP20P, total fructooligosaccharides were purified on Bio-Gel P-2 column eluted with water at the flow rate of 0.3. ml/min, which was the optimized conditions. The obtained fructooligosaccharides with degree of polymerization of 3-9 were identified based on their methylation analysis, MS and NMR data. This method has the advantages of high automation, good recovery and easy performance, which could be used for preparation of FOS from other sources, as well as other targeted compounds without UV absorbance. © 2013 Elsevier B.V.

KeywordBurdock Fplc Fructooligosaccharide Rid
DOI10.1016/j.chroma.2013.08.012
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000324353100006
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Cited Times [WOS]:16   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Jiangxi Science and Technology Normal University
2.University of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Li J.,Cheong K.L.,Zhao J.,et al. Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection[J]. Journal of Chromatography A,2013,1308:52-57.
APA Li J..,Cheong K.L..,Zhao J..,Hu D.J..,Chen X.Q..,...&Li S.P..(2013).Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection.Journal of Chromatography A,1308,52-57.
MLA Li J.,et al."Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection".Journal of Chromatography A 1308(2013):52-57.
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