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Simultaneous determination of seven hydrophilic bioactive compounds in water extract of Polygonum multiflorum using pressurized liquid extraction and short-end injection micellar electrokinetic chromatography
Lao K.-M.3; Han D.-Q.3; Chen X.-J.3; Zhao J.3; Wang T.-J.1; Li S.-P.3
2013-12-14
Source PublicationChemistry Central Journal
ISSN1752153X
Volume7Issue:1
Abstract

Background: Polygoni Multiflori Radix, He-Shou-Wu in Chinese, is a widely used traditional Chinese medicine. Clinically, water decoction is the major application form of He-Shou-Wu. Therefore, simultaneous determination of bioactive compounds in water extract is very important for its quality control. Results: A pressurized liquid extraction and short-end injection micellar electrokinetic chromatography (MEKC) were first developed for simultaneous determination of seven hydrophilic bioactive compounds in water extract of He-Shou-Wu. The influence of parameters, such as pH, concentration of phosphate, SDS and HP-β-CD, capillary temperature and applied voltage, on the analysis were carefully investigated. Optimum separation was obtained within 14 min by using 50 mM phosphate buffer containing 90 mM SDS and 2% (m/v) HP-β-CD (pH 2.5) at 15 kV and 20°C. All calibration curves showed good linearity (R >0.9978) within test ranges. The overall LOD and LOQ were lower than 2.0 μg/mL and 5.5 μg/mL, respectively. The RSDs for intra- and inter-day of seven analytes were less than 3.2% and 4.6%, and the recoveries were 97.0%-104.2%. Conclusion: The validated method was successfully applied to the analysis of He-Shou-Wu samples, which is helpful for its quality control.

KeywordMekc Polygonum Multiflorum Pressurized Liquid Extraction Short-end Injection
DOI10.1186/1752-153X-7-45
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000315958000001
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Shenzhen Institute for Drug Control
2.Jiangxi Science and Technology Normal University
3.University of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Lao K.-M.,Han D.-Q.,Chen X.-J.,et al. Simultaneous determination of seven hydrophilic bioactive compounds in water extract of Polygonum multiflorum using pressurized liquid extraction and short-end injection micellar electrokinetic chromatography[J]. Chemistry Central Journal,2013,7(1).
APA Lao K.-M.,Han D.-Q.,Chen X.-J.,Zhao J.,Wang T.-J.,&Li S.-P..(2013).Simultaneous determination of seven hydrophilic bioactive compounds in water extract of Polygonum multiflorum using pressurized liquid extraction and short-end injection micellar electrokinetic chromatography.Chemistry Central Journal,7(1).
MLA Lao K.-M.,et al."Simultaneous determination of seven hydrophilic bioactive compounds in water extract of Polygonum multiflorum using pressurized liquid extraction and short-end injection micellar electrokinetic chromatography".Chemistry Central Journal 7.1(2013).
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