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A simple analysis of fructooligosaccharides in two medicinal plants by high-performance thin-layer chromatography
Cheong K.-L.; Li J.; Zhao J.; Li S.-P.
2014-08-01
Source PublicationJournal of Planar Chromatography - Modern TLC
ISSN17890993 09334173
Volume27Issue:4Pages:245-250
AbstractA high-performance thin-layer chromatography (HPTLC) method for the analysis of seven fructooligosaccharides (FOS, DP3-9) in Morinda officinalis How. (M. officinalis) and Arctium lappa Linn. (A. lappa) has been established. The chromatographic analysis was developed twice with a mobile phase consisting of n-butanol-isopropanol-water-acetic acid in volume composition 7:5:2:1 and obtained on silica gel 60 plate. The chromatograms were visualized with 1-naphthol-sulfuric acid reagent. Densitometric detection was performed in visible light at 585 nm for seven FOS. The analytical range was set as 131-1050 ng for FOS. Calibration was linear within the selected range (R > 0.9901). The developed method was applied for the simultaneous determination of seven FOS in M. officinalis and A. lappa. The results indicated that the contents of FOS are variant in different M. officinalis samples, but constant in different A. lappa samples. This HPTLC method was found to be simple, stable, and reproducible. Hence, the developed HPTLC method would be an important tool for the quality control of FOS in medicinal plants.
KeywordArctium lappa Linn. Fructooligosaccharides High-performance thin-layer chromatography Morinda officinalis How.
DOI10.1556/JPC.27.2014.4.2
URLView the original
Language英語
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversity of Macau
First Author AffilicationUniversity of Macau
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GB/T 7714
Cheong K.-L.,Li J.,Zhao J.,et al. A simple analysis of fructooligosaccharides in two medicinal plants by high-performance thin-layer chromatography[J]. Journal of Planar Chromatography - Modern TLC,2014,27(4):245-250.
APA Cheong K.-L.,Li J.,Zhao J.,&Li S.-P..(2014).A simple analysis of fructooligosaccharides in two medicinal plants by high-performance thin-layer chromatography.Journal of Planar Chromatography - Modern TLC,27(4),245-250.
MLA Cheong K.-L.,et al."A simple analysis of fructooligosaccharides in two medicinal plants by high-performance thin-layer chromatography".Journal of Planar Chromatography - Modern TLC 27.4(2014):245-250.
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