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Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection
Zong W.-R.; Cheong K.-L.; Wu D.-T.; Li J.; Zhao J.; Li S.-P.
2014-12-10
Source PublicationSeparation and Purification Technology
ISSN13835866
Volume138Pages:98-103
AbstractRaffinose family oligosaccharides (RFOs) were extracted and isolated from Rehmannia glutinosa Libosch. by using microwave-assisted extraction and fast protein liquid chromatography coupled with refractive index detection (FPLC-RID). After extraction with 60% ethanol (v/v) and decolorization with MCI gel CHP20P, total oligosaccharides were purified by using FPLC-RID coupled with the Bio-Gel P2 column eluted with water at the flow rate of 0.3 mL/min. Three pure oligosaccharides (DP3-DP5) were obtained with the yields of 1.72%, 7.52% and 0.51%, respectively, as well as identified as raffinose, stachyose and verbascose from the results of liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS) and gas chromatography coupled with mass spectroscopy (GC-MS) analysis. A large-scale of pure RFOs was automatically and efficiently separated from R. glutinosa by using FPLC-RID method, which was beneficial for the qualitative and quantitative analysis of RFOs in R. glutinosa and their products.
KeywordFPLC-RID GC-MS LC-MS/MS Raffinose family oligosaccharides Rehmannia glutinosa Libosch.
DOI10.1016/j.seppur.2014.10.001
URLView the original
Language英語
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversity of Macau
First Author AffilicationUniversity of Macau
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GB/T 7714
Zong W.-R.,Cheong K.-L.,Wu D.-T.,et al. Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection[J]. Separation and Purification Technology,2014,138:98-103.
APA Zong W.-R.,Cheong K.-L.,Wu D.-T.,Li J.,Zhao J.,&Li S.-P..(2014).Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection.Separation and Purification Technology,138,98-103.
MLA Zong W.-R.,et al."Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection".Separation and Purification Technology 138(2014):98-103.
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