UM
Analysis of Cordyceps by multi-column liquid chromatography
Qian Z.2; Li S.2
2017-03-01
Source PublicationActa Pharmaceutica Sinica B
ISSN22113843 22113835
Volume7Issue:2Pages:202-207
AbstractCordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps. The MC-LC system includes a size exclusion pre-column, a size exclusion column (SEC) and a reversed phase column (RP) which were controlled by column-switching valves. The sample was separated by the size exclusion pre-column into two fractions (macromolecules and micromolecules). These fractions were further separated on SEC and RP columns, respectively. A diode array detector (DAD) and a mass spectrometer (MS) were used to detect the components. This MC-LC method was utilized for analysis of Cordyceps samples. Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps, and 11 of the micromolecular peaks were identified as adenosine-5′-monophosphate (AMP), phenylalanine, uridine, hypoxanthine, inosine, guanine, guanosine, deoxyadenosine-5′-monophosphate (dAMP), adenosine, adenine and cordycepin (or its isomer). This method is useful for quality control of Cordyceps.
KeywordCordyceps Macromolecules Micromolecules Multi-column Size exclusion column
DOI10.1016/j.apsb.2016.10.002
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Sunshine Lake Pharma Co., Ltd
2.University of Macau
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Qian Z.,Li S.. Analysis of Cordyceps by multi-column liquid chromatography[J]. Acta Pharmaceutica Sinica B,2017,7(2):202-207.
APA Qian Z.,&Li S..(2017).Analysis of Cordyceps by multi-column liquid chromatography.Acta Pharmaceutica Sinica B,7(2),202-207.
MLA Qian Z.,et al."Analysis of Cordyceps by multi-column liquid chromatography".Acta Pharmaceutica Sinica B 7.2(2017):202-207.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qian Z.]'s Articles
[Li S.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qian Z.]'s Articles
[Li S.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qian Z.]'s Articles
[Li S.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.