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Flavonoids in natural and tissue cultured materials of Epimedium alpinum identified by using UHPLC-Q-TOF-MS/MS
Pilepic K.H.1; Yang Z.2; Chen J.2; Chen X.2; Wang Y.2; Zhao J.2; Mihaljevic S.1; Li S.P.2
Source PublicationInternational Journal of Mass Spectrometry
AbstractHerbra Epimedii is a common and widely used traditional Chinese medicine for thousands of years. Epimedium alpinum L. is one of the representative herb of Epimedium species in Europe, which is used as a diaphoretic and applied in breast illness in folk medicine. In this paper, an efficient UHPLC-Q-TOF-MS method was established for rapid discovery and identification of prenylated flavonoid glucosides in E. alpinum from Croatia. Based on the MS fragment pathways, rules and intensity of 12 reference compounds in negative and positive ion mode, the kinds of substituents and site of saccharides linkage of 79 prenylated flavonoid glucosides were tentatively characterized. Different substituents at C-3 and C-4 in the ring B formed four skeletons, including skeleton A with hydrogen and hydroxyl, skeleton B with hydrogen and methoxyl, skeleton C with hydroxyl and methoxyl and skeleton D with two hydroxyls, were identified in E. alpinum. Based on our knowledge, the skeleton D was first identified in herb medicine and 23 new compounds with skeleton D were characterized. According to these findings, prenylated flavonoids glucosides of 40 leaves, rhizomes, buds and tissue cultural products of E. alpinum were further systematically investigated. Chemicals in E. alpinum from Croatia were obviously different from those in E. koreanum Nakai and E. brevicomu Maxim from China via principal component analysis (PCA). This study provide an effective strategy for new compounds discovery in herbal extract, which is helpful to quality control and pharmacological evaluation of E. alpinum from Croatia.
KeywordEpimedium alpinum L. PCA Prenylated flavonoids glucosides Structural characterization UHPLC-Q-TOF-MS
URLView the original
Indexed BySCI
WOS Research AreaPhysics ; Spectroscopy
WOS SubjectPhysics, Atomic, Molecular & Chemical ; Spectroscopy
WOS IDWOS:000449709900032
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Institute Ruder Boskovic
2.University of Macau
Recommended Citation
GB/T 7714
Pilepic K.H.,Yang Z.,Chen J.,et al. Flavonoids in natural and tissue cultured materials of Epimedium alpinum identified by using UHPLC-Q-TOF-MS/MS[J]. International Journal of Mass Spectrometry,2018,434:222-232.
APA Pilepic K.H..,Yang Z..,Chen J..,Chen X..,Wang Y..,...&Li S.P..(2018).Flavonoids in natural and tissue cultured materials of Epimedium alpinum identified by using UHPLC-Q-TOF-MS/MS.International Journal of Mass Spectrometry,434,222-232.
MLA Pilepic K.H.,et al."Flavonoids in natural and tissue cultured materials of Epimedium alpinum identified by using UHPLC-Q-TOF-MS/MS".International Journal of Mass Spectrometry 434(2018):222-232.
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