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Characterization of chenodeoxycholic acid as an endogenous antagonist of the G-coupled formyl peptide receptors
Chen X.; Yang D.; Shen W.; Dong H.F.; Wang J.M.; Oppenheim J.J.; Howard O.M.Z.
2000-12-01
Source PublicationInflammation Research
ISSN10233830
Volume49Issue:12Pages:744-755
Abstract

Objective and design: To demonstrate the role of bile acids in immune modulation we examined the ability of select bile acids to inhibit leukocyte migration and chemoattractant receptor function. Materials: To elucidate this mechanism, we employed primary human monocytes, neutrophils and cell lines transfected to express either the high affinity fMLP receptor (FPR) or the low affinity fMLP receptor like 1 (FPRL1). Treatment: Cells were treated with chenodeoxycholic acid (CDCA) and related bile acids in a 0-400 micromolar range. Method: Cell viability, chemotaxis and calcium flux analysis were preformed. Results: We observed that pathophysiological levels (≤150 micromolar) of CDCA competitively inhibited H-fMLP binding to human monocytes, FPR and FPRL1 transfected cells. Additionally, CDCA reduced both the chemotactic and calcium flux responses induced by fMLP or "W" peptide. Further, CDCA inhibited anti-FPR antibody binding to monocytes. Conclusions: CDCA selectively inhibited human leukocyte chemotaxis and calcium flux induced by fMLP, but not other chemoattractants, suggesting a mechanism for inhibition of inflammation and suppression of innate immune response.

KeywordBile Acids Chemotaxis Chenodeoxycholic Acid Formyl Peptide Receptor Formyl Peptide Receptor Like 1. Calcium Flux
DOI10.1007/s000110050656
URLView the original
Indexed BySCI
WOS Research AreaCell Biology ; Immunology
WOS SubjectCell Biology ; Immunology
WOS IDWOS:000166814100015
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Cited Times [WOS]:37   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationNational Cancer Institute at Frederick
Recommended Citation
GB/T 7714
Chen X.,Yang D.,Shen W.,et al. Characterization of chenodeoxycholic acid as an endogenous antagonist of the G-coupled formyl peptide receptors[J]. Inflammation Research,2000,49(12):744-755.
APA Chen X..,Yang D..,Shen W..,Dong H.F..,Wang J.M..,...&Howard O.M.Z..(2000).Characterization of chenodeoxycholic acid as an endogenous antagonist of the G-coupled formyl peptide receptors.Inflammation Research,49(12),744-755.
MLA Chen X.,et al."Characterization of chenodeoxycholic acid as an endogenous antagonist of the G-coupled formyl peptide receptors".Inflammation Research 49.12(2000):744-755.
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