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Regulatory effects of deoxycholic acid, a component of the anti-inflammatory traditional Chinese medicine Niuhuang, on human leukocyte response to chemoattractants
Chen X.2; Mellon R.D.2; Yang L.2; Dong H.1; Oppenheim J.J.2; Howard O.M.Z.2
2002-02-01
Source PublicationBiochemical Pharmacology
ISSN00062952
Volume63Issue:3Pages:533-541
Abstract

Niuhuang is a commonly used Chinese traditional medicine with immunoregulatory and anti-inflammatory properties. Deoxycholic acid (DCA) is a major active constituent of Niuhuang. The reaction of human leukocytes to chemoattractants is an important part of the host immune response and also plays a crucial role in the development of inflammation. We, therefore, investigated the in vitro effects of DCA on human monocyte and neutrophil responses to classic chemoattractants [fMet-Leu-Phe (fMLP), complement fraction 5a (C5a)], CC chemokine [monocyte chemoattractant protein-1 (MCP-1/CCL2)], and/or CXC chemokines [stromal cell-derived factor-1 (SDF-1α/CXCL12), interleukin-8 (IL-8/CXCL8)]. The results showed that DCA significantly inhibited fMLP-induced monocyte and neutrophil chemotaxis and calcium mobilization, and also blocked the binding of [H]fMLP and anti-formyl peptide receptor (FPR) monoclonal antibodies (mAb) to the cells. The inhibitory effects of DCA on calcium mobilization and anti-FPR-mAb binding to the receptor could be abrogated by washing DCA out of the cell suspension, suggesting that DCA blocked fMLP receptors via a steric hindrance mechanism, not via receptor internalization. DCA had no significant inhibitory effects on MCP-1-, SDF-1α-, or C5a-induced monocyte function, or C5a- or IL-8-induced neutrophil function. Taken together, our experimental results suggest that blockade of fMLP receptors may contribute to the anti-inflammatory effects of traditional medicine containing DCA. © 2002 Elsevier Science Inc. All rights reserved.

KeywordCalcium Mobilization Chemokine/chemotaattractant Chemotaxis Deoxycholic Acid (Dca) Human Leukocyte Ligand-receptor Binding
DOI10.1016/S0006-2952(01)00917-0
URLView the original
Indexed BySCI
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000174506900020
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Cited Times [WOS]:22   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.SAIC-Frederick
2.National Cancer Institute at Frederick
Recommended Citation
GB/T 7714
Chen X.,Mellon R.D.,Yang L.,et al. Regulatory effects of deoxycholic acid, a component of the anti-inflammatory traditional Chinese medicine Niuhuang, on human leukocyte response to chemoattractants[J]. Biochemical Pharmacology,2002,63(3):533-541.
APA Chen X.,Mellon R.D.,Yang L.,Dong H.,Oppenheim J.J.,&Howard O.M.Z..(2002).Regulatory effects of deoxycholic acid, a component of the anti-inflammatory traditional Chinese medicine Niuhuang, on human leukocyte response to chemoattractants.Biochemical Pharmacology,63(3),533-541.
MLA Chen X.,et al."Regulatory effects of deoxycholic acid, a component of the anti-inflammatory traditional Chinese medicine Niuhuang, on human leukocyte response to chemoattractants".Biochemical Pharmacology 63.3(2002):533-541.
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