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Effects of Shuanghuanglian and Qingkailing, two multi-components of traditional Chinese medicinal preparations, on human leukocyte function
Chen X.2; Zack Howard O.M.2; Yang X.1; Wang L.1; Oppenheim J.J.2; Krakauer T.3
2002-05-03
Source PublicationLife Sciences
ISSN00243205
Volume70Issue:24Pages:2897-2913
Abstract

Qingkailing (QKL) and Shuanghuanglian (SHHL) are two commonly used Chinese herbal preparations with reported antiinflammatory activity. The effects of these two preparations on the capacity of staphylococcal toxic shock syndrome toxin 1 (TSST-1) to stimulate the production of cytokines (IL-1β, IL-6, TNF-α, IFN-γ) and chemokines (MIP-1α, MIP-1β and MCP-1) by peripheral blood mononuclear cell (PBMC) was tested. We also evaluated their effect on LPS-stimulated NF-κB transcriptional activity in a THP-1 cell line, and on human monocyte chemotactic response to chemoattractants. Non-cytotoxic concentrations of QKL (0.1∼2%) and SHHL (6∼120 μg) significantly inhibited production of cytokines and chemokines in a dose-dependent manner (P < 0.05). Both, QKL at 1:100 and SHHL at 60 μg/ml, markedly inhibited RANTES, MIP-1α, SDF-1α and fMLP induced human monocyte migration (P < 0.05 or 0.01). QKL (1%) did not inhibit monocyte chemotaxis induced by super-or sub-optimal concentrations of fMLP (10, 10 and 10 M), but only inhibited chemotaxis induced by optimal concentrations of fMLP at 10, 10 and 10 M. QKL (0.1% or 1%) and SHHL(6 or 60 μg/ml) markedly inhibited LPS-induced NF-κB activity in THP-1 cells. The results suggested that the pharmacological basis for the antiinflammatory effects of QKL and SHHL is the result of suppression of NF-κB regulated gene transcription, leading to suppressed production of proinflammatory cytokine and chemokine. Interference with leukocyte chemotaxis also contributes to the antiinflammatory and immunomodulating effects of these medicinals. Identification of the responsible components in these two herbal preparations may yield compounds suitable for structural modification into potent novel drugs. © 2002 Elsevier Science Inc. All rights reserved.

KeywordChemokine Chemotaxis Cytokine Nf-κb Qingkailing (Qkl) Shuanghuanglian (Shhl)
DOI10.1016/S0024-3205(02)01541-2
URLView the original
Indexed BySCI
WOS Research AreaResearch & Experimental Medicine ; Pharmacology & Pharmacy
WOS SubjectMedicine, Research & Experimental ; Pharmacology & Pharmacy
WOS IDWOS:000176374800005
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Cited Times [WOS]:37   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.SAIC-Frederick
2.National Cancer Institute at Frederick
3.U.S. Army Medical Research Institute of Infectious Diseases
Recommended Citation
GB/T 7714
Chen X.,Zack Howard O.M.,Yang X.,et al. Effects of Shuanghuanglian and Qingkailing, two multi-components of traditional Chinese medicinal preparations, on human leukocyte function[J]. Life Sciences,2002,70(24):2897-2913.
APA Chen X.,Zack Howard O.M.,Yang X.,Wang L.,Oppenheim J.J.,&Krakauer T..(2002).Effects of Shuanghuanglian and Qingkailing, two multi-components of traditional Chinese medicinal preparations, on human leukocyte function.Life Sciences,70(24),2897-2913.
MLA Chen X.,et al."Effects of Shuanghuanglian and Qingkailing, two multi-components of traditional Chinese medicinal preparations, on human leukocyte function".Life Sciences 70.24(2002):2897-2913.
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