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RNase protection assay for the study of the differential effects of therapeutic agents in suppressing staphylococcal enterotoxin B-induced cytokines in human peripheral blood mononuclear cells.
Krakauer T.1; Chen X.1; Howard O.M.1; Young H.A.2
2003-12-01
Source PublicationMethods in molecular biology (Clifton, N.J.)
PublisherSpringer
Pages151-164
Abstract

Staphylococcal exotoxins (SE) are among the most common etiological agents that cause toxic shock (1). Similar to other superantigens, SE activates T cells polyclonally by binding simultaneously to specific Vβ regions of T-cell receptors (TCR) on T cells and the major histocompatibility complex class II (MHC II) molecules on antigen-presenting cells (APCs) (2,3). Interaction of SE with TCR and MHC II results in a massive release of proinflammatory cytokines from stimulated cells (4,5). Previous studies demonstrated that the cytokines produced, tumor-necrosis factor (TNF-α), interleukin 1 (IL-1), and interferon-γ (IFN-γ) are pivotal mediators in SE-induced toxic shock (6,7). High levels of these cytokines in serum are pathogenic and correlate with clinical symptoms of toxic shock (8,10).

KeywordMajor Histocompatibility Complex Class Chlorogenic Acid Hybridization Buffer Human Peripheral Blood Mononuclear Cell Rnase Protection Assay
URLView the original
ISBN1064-3745
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Document TypeBook chapter
专题Institute of Chinese Medical Sciences
Affiliation1.Laboratory of Molecular ImmunoregulationNational Cancer InstituteFrederick
2.Laboratory of Experimental ImmunologyNational Cancer InstituteFrederick
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GB/T 7714
Krakauer T.,Chen X.,Howard O.M.,et al. RNase protection assay for the study of the differential effects of therapeutic agents in suppressing staphylococcal enterotoxin B-induced cytokines in human peripheral blood mononuclear cells.:Springer,2003:151-164.
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