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Pertussis toxin as an adjuvant suppresses the number and function of CD4+CD25+ T regulatory cells
Chen X.2; Winkler-Pickett R.T.1; Carbonetti N.H.3; Ortaldo J.R.1; Oppenheim J.J.1; Howard O.M.Z.1
2006-03-01
Source PublicationEuropean Journal of Immunology
ISSN00142980
Volume36Issue:3Pages:671-680
Abstract

We observed a remarkable reduction in the frequency and immunosuppressive activity of splenic CD4CD25 T cells in C57BL/6 mice with MOG-induced experimental autoimmune encephalomyelitis (EAE). Our study revealed that pertussis toxin (PTx), one component of the immunogen used to induce murine EAE, was responsible for down-regulating splenic CD4CD25 cells. Treatment of normal BALB/c mice with PTx in vivo reduced the frequency, suppressive activity and FoxP3 expression by splenic CD4CD25 T cells. However, PTx treatment did not alter the expression of characteristic phenotypic markers (CD45RB, CD103, GITR and CTLA-4) and did not increase the expression of CD44 and CD69 by the residual splenic and lymph node CD4CD25 T cells. This property of PTx was attributable to its ADP-ribosyltransferase activity. PTx did not inhibit suppressive activity of purified CD4CD25 T regulatory (Treg) cells in vitro, but did so in vivo, presumably due to an indirect effect. Although the exact molecular target of PTx that reduces Treg activity remains to be defined, our data suggests that alteration of both distribution and function of splenic immunocytes should play a role. This study concludes that an underlying cause for the immunological adjuvanticity of PTx is down-regulation of Treg cell number and function. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywordcd4+cd25+ t Regulatory Cells Experimental Autoimmune Encephalomyelitis Pertussis Toxin
DOI10.1002/eji.200535353
URLView the original
Indexed BySCI
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000236457400017
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Cited Times [WOS]:83   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.National Cancer Institute at Frederick
2.SAIC-Frederick
3.University of Maryland School of Medicine
Recommended Citation
GB/T 7714
Chen X.,Winkler-Pickett R.T.,Carbonetti N.H.,et al. Pertussis toxin as an adjuvant suppresses the number and function of CD4+CD25+ T regulatory cells[J]. European Journal of Immunology,2006,36(3):671-680.
APA Chen X.,Winkler-Pickett R.T.,Carbonetti N.H.,Ortaldo J.R.,Oppenheim J.J.,&Howard O.M.Z..(2006).Pertussis toxin as an adjuvant suppresses the number and function of CD4+CD25+ T regulatory cells.European Journal of Immunology,36(3),671-680.
MLA Chen X.,et al."Pertussis toxin as an adjuvant suppresses the number and function of CD4+CD25+ T regulatory cells".European Journal of Immunology 36.3(2006):671-680.
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