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Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
Ho Yan Gloria Tse1; Michelle Nga Yu Hui1; Li Li1; Simon Ming Yuen Lee3; Anskar Yu Hung Leung2; Shuk Han Cheng1
2012-01-31
Source PublicationJournal of Ethnopharmacology
ISSN03788741 18727573
Volume139Issue:2Pages:447-453
Abstract

Ethnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration.

KeywordAngiogenesis Diabetic Foot Ulcer Fgf Mapk Radix Astragali Radix Rehmanniae Vegf Wound Healing Zebrafish Embryos
DOI10.1016/j.jep.2011.11.031
URLView the original
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000302505900018
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorShuk Han Cheng
Affiliation1.Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong Special Administrative Region
2.Division of Haematology and Bone Marrow Transplantation, Department of Medicine, Queen Mary Hospital, University of Hong Kong, Pok Fu Lam Road, Hong Kong Special Administrative Region
3.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomas Pereira, Taipa, Macau
Recommended Citation
GB/T 7714
Ho Yan Gloria Tse,Michelle Nga Yu Hui,Li Li,et al. Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro[J]. Journal of Ethnopharmacology,2012,139(2):447-453.
APA Ho Yan Gloria Tse,Michelle Nga Yu Hui,Li Li,Simon Ming Yuen Lee,Anskar Yu Hung Leung,&Shuk Han Cheng.(2012).Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro.Journal of Ethnopharmacology,139(2),447-453.
MLA Ho Yan Gloria Tse,et al."Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro".Journal of Ethnopharmacology 139.2(2012):447-453.
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