Neuroprotective effects of the andrographolide analogue AL-1 in the MPP+/MPTP-induced Parkinson's disease model in vitro and in mice | |
Zhang Z.3; Lai D.3; Wang L.1; Yu P.3; Zhu L.3; Guo B.3; Xu L.3; Zhou L.2; Sun Y.3; Lee S.M.Y.1![]() | |
2014 | |
Source Publication | Pharmacology Biochemistry and Behavior
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ISSN | 18735177 00913057 |
Volume | 122Pages:191-202 |
Abstract | The andrographolide-lipoic acid conjugate AL-1 is a newly synthesized molecule by covalently linking andrographolide (Andro) with α-lipoic acid (LA). In the present work, the neuroprotective effect of AL-1 was investigated in vitro and in a mouse model of the Parkinson's disease (PD). We found that AL-1 significantly prevented 1-methyl-4-phenylpyridinium (MPP )-induced neurotoxicity in SH-SY5Y cells and primary cerebellar granule neurons. In a mouse model of Parkinson's disease, AL-1 rescued 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced loss of tyrosine hydroxylase (TH)-positive neurons, improved the behavioral impairment, and elevated the striatal levels of dopamine and its metabolites 3,4-dihydroxyphenylacetic acid. Furthermore, AL-1 remarkably lowered the nitric oxide and malondialdehyde levels while increased the superoxide dismutase level in the substantial nigra of MPTP-treated mice. The immunoblotting data showed that AL-1 significantly ameliorated the decreased expression of TH protein in the substantial nigra and inhibited the up-regulation of phosphorylated NF-κB p65 in vitro and in vivo. Taken together, AL-1 exerted neuroprotective effect in vitro and in animal model of PD through anti-oxidation and inhibition of NF-κB activation. © 2014 Elsevier Inc. All rights reserved. |
Keyword | Andrographolide-lipoic Acid Conjugate Neuroprotection Nf-κb P65 Parkinson's Disease |
DOI | 10.1016/j.pbb.2014.03.028 |
URL | View the original |
Indexed By | SCI |
WOS Research Area | Behavioral Sciences ; Neurosciences & Neurology ; Pharmacology & Pharmacy |
WOS Subject | Behavioral Sciences ; Neurosciences ; Pharmacology & Pharmacy |
WOS ID | WOS:000338597000022 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Institute of Chinese Medical Sciences |
Affiliation | 1.University of Macau 2.Jinan University 3.Jinan University |
Recommended Citation GB/T 7714 | Zhang Z.,Lai D.,Wang L.,et al. Neuroprotective effects of the andrographolide analogue AL-1 in the MPP+/MPTP-induced Parkinson's disease model in vitro and in mice[J]. Pharmacology Biochemistry and Behavior,2014,122:191-202. |
APA | Zhang Z..,Lai D..,Wang L..,Yu P..,Zhu L..,...&Wang Y..(2014).Neuroprotective effects of the andrographolide analogue AL-1 in the MPP+/MPTP-induced Parkinson's disease model in vitro and in mice.Pharmacology Biochemistry and Behavior,122,191-202. |
MLA | Zhang Z.,et al."Neuroprotective effects of the andrographolide analogue AL-1 in the MPP+/MPTP-induced Parkinson's disease model in vitro and in mice".Pharmacology Biochemistry and Behavior 122(2014):191-202. |
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