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Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways
Lei S.W.2; Cui G.2; Leung G.P.H.3; Luk S.C.W.2; Hoi M.P.M.2; Wang L.2; Mahady G.B.1; Lee S.M.Y.2
2015
Source PublicationJournal of Functional Foods
ISSN17564646
Volume18Pages:213-223
AbstractIcaritin, a prenylflavonoid isolated from Herbal Epimedii, is well known for its osteoplastic and anti-tumour activities. Here, we examined the cardioprotective actions of icaritin on cardiac H9c2 cells under oxidative stress conditions induced by treatment with tert-butylhydroperoxide (t-BHP). Icaritin at a concentration range between 1 and 4 μM effectively increased cell viability and decreased lactate dehydrogenase (LDH) leakage and reactive oxygen species (ROS) release in t-BHP treated cells, reflecting its ability to reduce t-BHP-induced cell injury. Icaritin also protected cell membrane integrity by preventing the collapse of the mitochondrial membrane potential. In addition, pretreatment of NF-E2-related factor 2 (Nrf2) siRNA and Akt inhibitor abolished the cardioprotective effect of icaritin. Furthermore, Nrf2 siRNA transfection interfered with the effect of icaritin on the inhibition of Ca2+ overload, whereas Akt inhibitor reduced icaritin-induced haemo-oxygenase-1 (HO-1) expression. Collectively, these results suggest that icaritin exerted its cardioprotective effect through the Akt/Nrf2/HO-1 and calcium signalling pathways.
KeywordCalcium ion Cardiac H9c2 cells Icaritin NF-E2-related factor 2 Oxidative stress
DOI10.1016/j.jff.2015.06.054
URLView the original
Language英語
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.University of Illinois at Chicago
2.University of Macau
3.The University of Hong Kong Li Ka Shing Faculty of Medicine
First Author AffilicationUniversity of Macau
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GB/T 7714
Lei S.W.,Cui G.,Leung G.P.H.,et al. Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways[J]. Journal of Functional Foods,2015,18:213-223.
APA Lei S.W..,Cui G..,Leung G.P.H..,Luk S.C.W..,Hoi M.P.M..,...&Lee S.M.Y..(2015).Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways.Journal of Functional Foods,18,213-223.
MLA Lei S.W.,et al."Icaritin protects against oxidative stress-induced injury in cardiac H9c2 cells via Akt/Nrf2/HO-1 and calcium signalling pathways".Journal of Functional Foods 18(2015):213-223.
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