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In vitro Vitamin K2 and 1α,25-dihydroxyvitamin D3 combination enhances osteoblasts anabolism of diabetic mice
Christina C.W. Poon1; Rachel W.S. Li1,2; Sai Wang Seto7; Siu Kai Kong4; Ho Pui Ho5; Maggie P.M. Hoi6; Simon M.Y. Lee6; Sai Ming Ngai4; Shun Wan Chan3; George P.H. Leung2; Yiu Wa Kwan1
2015-11-15
Source PublicationEuropean Journal of Pharmacology
ISSN18790712 00142999
Volume767Pages:30-40
Abstract

In this study, we evaluated the anabolic effect and the underlying cellular mechanisms involved of vitamin K (10 nM) and 1α,25-dihydroxyvitamin D (1,25(OH)D) (10 nM), alone and in combination, on primary osteoblasts harvested from the iliac crests of C57BL/KsJ lean (+/+) and obese/diabetic (db/db) mice. A lower alkaline phosphatase (ALP) activity plus a reduced expression of bone anabolic markers and bone formation transcription factors (osteocalcin, Runx2, Dlx5, ATF4 and OSX) were consistently detected in osteoblasts of db/db mice compared to lean mice. A significantly higher calcium deposits formation in osteoblasts was observed in lean mice when compared to db/db mice. Co-administration of vitamin K (10 nM) and 1,25(OH)D (10 nM) caused an enhancement of calcium deposits in osteoblasts in both strains of mice. Vitamins K and 1,25(OH)D co-administration time-dependently (7, 14 and 21 days) increased the levels of bone anabolic markers and bone formation transcription factors, with a greater magnitude of increase observed in osteoblasts of db/db mice. Combined vitamins K plus 1,25(OH)D treatment significantly enhanced migration and the re-appearance of surface microvilli and ruffles of osteoblasts of db/db mice. Thus, our results illustrate that vitamins K plus D combination could be a novel therapeutic strategy in treating diabetes-associated osteoporosis.

Keyword1,25(Oh)2d3 Osteoblasts Osteoporosis Type 2 Diabetes Mellitus Vitamin K2
DOI10.1016/j.ejphar.2015.09.048
URLView the original
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000364839500005
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorGeorge P.H. Leung; Yiu Wa Kwan
Affiliation1.School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong
2.Department of Pharmacology and Pharmacy, Faculty of Medicine, The University of Hong Kong, Hong Kong
3.State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong
4.School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Hong Kong
5.Department of Electronic Engineering, Faculty of Engineering, The Chinese University of Hong Kong, Hong Kong
6.Institute of Chinese Medical Sciences, The University of Macau, Macau, China
7.National Institute of Complementary Medicine, School of Science and Health, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia
Recommended Citation
GB/T 7714
Christina C.W. Poon,Rachel W.S. Li,Sai Wang Seto,et al. In vitro Vitamin K2 and 1α,25-dihydroxyvitamin D3 combination enhances osteoblasts anabolism of diabetic mice[J]. European Journal of Pharmacology,2015,767:30-40.
APA Christina C.W. Poon.,Rachel W.S. Li.,Sai Wang Seto.,Siu Kai Kong.,Ho Pui Ho.,...&Yiu Wa Kwan.(2015).In vitro Vitamin K2 and 1α,25-dihydroxyvitamin D3 combination enhances osteoblasts anabolism of diabetic mice.European Journal of Pharmacology,767,30-40.
MLA Christina C.W. Poon,et al."In vitro Vitamin K2 and 1α,25-dihydroxyvitamin D3 combination enhances osteoblasts anabolism of diabetic mice".European Journal of Pharmacology 767(2015):30-40.
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