UM
Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2T from Lop Nor region, China
Huang C.1; Leung R.K.-K.2; Guo M.1; Tuo L.3; Guo L.3; Yew W.W.2; Lou I.1; Lee S.M.Y.1; Sun C.3
2016-02-11
Source PublicationScientific Reports
ISSN20452322
Volume6
AbstractMicrobial secondary metabolites are valuable resources for novel drug discovery. In particular, actinomycetes expressed a range of antibiotics against a spectrum of bacteria. In genus level, strain Allosalinactinospora lopnorensis CA15-2 is the first new actinomycete isolated from the Lop Nor region, China. Antimicrobial assays revealed that the strain could inhibit the growth of certain types of bacteria, including Acinetobacter baumannii and Staphylococcus aureus, highlighting its clinical significance. Here we report the 5,894,259 base pairs genome of the strain, containing 5,662 predicted genes, and 832 of them cannot be detected by sequence similarity-based methods, suggesting the new species may carry a novel gene pool. Furthermore, our genome-mining investigation reveals that A. lopnorensis CA15-2 contains 17 gene clusters coding for known or novel secondary metabolites. Meanwhile, at least six secondary metabolites were disclosed from ethyl acetate (EA) extract of the fermentation broth of the strain by high-resolution UPLC-MS. Compared with reported clusters of other species, many new genes were found in clusters, and the physical chromosomal location and order of genes in the clusters are distinct. This study presents evidence in support of A. lopnorensis CA15-2 as a potent natural products source for drug discovery.
DOI10.1038/srep20667
URLView the original
Language英語
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.University of Macau
2.Chinese University of Hong Kong
3.Chinese Academy of Medical Sciences
4.The University of Hong Kong
First Author AffilicationUniversity of Macau
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GB/T 7714
Huang C.,Leung R.K.-K.,Guo M.,et al. Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2T from Lop Nor region, China[J]. Scientific Reports,2016,6.
APA Huang C..,Leung R.K.-K..,Guo M..,Tuo L..,Guo L..,...&Sun C..(2016).Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2T from Lop Nor region, China.Scientific Reports,6.
MLA Huang C.,et al."Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2T from Lop Nor region, China".Scientific Reports 6(2016).
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