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A novel non-viral gene vector for hepatocyte-targeting and: In situ monitoring of DNA delivery in single cells
Gao Y.-G.1; Tang Q.1; Shi Y.-D.1; Zhang Y.1; Wang R.2; Lu Z.-L.1
2016
Source PublicationRSC Advances
ISSN20462069
Volume6Issue:55Pages:50053-50060
AbstractA macrocyclic polyamine lipid bearing both fluorescent naphthalimide and targeting cholic acid moieties, 1, was designed and synthesized as a novel non-viral gene vector. Plasmid DNA was completely condensed at 10 μM of 1 (N/P = 3.6) in 5 minutes at 37 °C. The process of DNA uptake process by 1 in a single cell was clearly investigated through time-dependent fluorescence microscopy assay. Flow cytometry experiments of liposome 1 clearly proved its targeting activity toward HepG2 cells. Luciferase gene expression transfected by the DNA complex of 1/DOPE gave a higher transfection efficiency in HepG2, A549, HeLa, U2OS and CCC-HPF-1 cells than by lipofectamine 2000. The present work demonstrated that the designed multifunctional organic molecule is able to fluorescently track the gene delivery process at a single-cell level for the first time. This will greatly aid mechanistic studies of gene transfection and further development of new gene vectors for biomedical applications.
DOI10.1039/c6ra08935f
URLView the original
Language英語
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Beijing Normal University
2.University of Macau
Recommended Citation
GB/T 7714
Gao Y.-G.,Tang Q.,Shi Y.-D.,et al. A novel non-viral gene vector for hepatocyte-targeting and: In situ monitoring of DNA delivery in single cells[J]. RSC Advances,2016,6(55):50053-50060.
APA Gao Y.-G.,Tang Q.,Shi Y.-D.,Zhang Y.,Wang R.,&Lu Z.-L..(2016).A novel non-viral gene vector for hepatocyte-targeting and: In situ monitoring of DNA delivery in single cells.RSC Advances,6(55),50053-50060.
MLA Gao Y.-G.,et al."A novel non-viral gene vector for hepatocyte-targeting and: In situ monitoring of DNA delivery in single cells".RSC Advances 6.55(2016):50053-50060.
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