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Simultaneous Quantification of Three Curcuminoids and Three Volatile Components of Curcuma longa Using Pressurized Liquid Extraction and High-Performance Liquid Chromatography
Chao, In-Cheng1; Wang, Chun-Ming1; Li, Shao-Ping1; Lin, Li-Gen1; Ye, Wen-Cai2; Zhang, Qing-Wen1
2018-07
Source PublicationMOLECULES
ISSN1420-3049
Volume23Issue:7
Abstract

A high-performance liquid chromatography (HPLC) method was investigated for the simultaneous quantification of two chemical types of bioactive compounds in the rhizome of Curcuma longa Linn. (turmeric), including three curcuminoids: Curcumin, bisdemethoxycurcumin, and demethoxycurcumin; and three volatile components: ar-turmerone, beta-turmerone, and alpha-turmerone. In the present study, the sample extraction system was optimized by a pressurized liquid extraction (PLE) process for further HPLC analysis. The established HPLC analysis conditions were achieved using a Zorbax SB-C18 column (250 mm x 4.6 mm i.d., 5 mu m) and a gradient mobile phase comprised of acetonitrile and 0.4% (v/v) aqueous acetic acid with an eluting rate of 1.0 mL/min. The curcuminoids and volatile components were detected at 430 nm and 240 nm, respectively. Moreover, the method was validated in terms of linearity, sensitivity, precision, stability and accuracy. The validated method was successfully applied to evaluate the quality of twelve commercial turmeric samples.

KeywordHplc Curcuma Longa Turmeric Curcuminoids Turmerone Quantification
DOI10.3390/molecules23071568
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000445301800071
PublisherMDPI
The Source to ArticleWOS
Citation statistics
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhang, Qing-Wen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China
2.Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chao, In-Cheng,Wang, Chun-Ming,Li, Shao-Ping,et al. Simultaneous Quantification of Three Curcuminoids and Three Volatile Components of Curcuma longa Using Pressurized Liquid Extraction and High-Performance Liquid Chromatography[J]. MOLECULES,2018,23(7).
APA Chao, In-Cheng,Wang, Chun-Ming,Li, Shao-Ping,Lin, Li-Gen,Ye, Wen-Cai,&Zhang, Qing-Wen.(2018).Simultaneous Quantification of Three Curcuminoids and Three Volatile Components of Curcuma longa Using Pressurized Liquid Extraction and High-Performance Liquid Chromatography.MOLECULES,23(7).
MLA Chao, In-Cheng,et al."Simultaneous Quantification of Three Curcuminoids and Three Volatile Components of Curcuma longa Using Pressurized Liquid Extraction and High-Performance Liquid Chromatography".MOLECULES 23.7(2018).
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