UM  > 中華醫藥研究院
PXR-Mediated Upregulation of CYP3A Expression by Herb Compound Praeruptorin C from Peucedanum praeruptorum Dunn
Huang, Ling1,2; Wu, Qian2; Li, Yu-Hua1; Wang, Yi-Tao3; Bi, Hui-Chang1
2013
Source PublicationEVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
ISSN1741-427X
Abstract

We recently reported that Praeruptorin C effectively transactivated the mRNA, protein expression, and catalytic activity of CYP3A4 via the CAR-mediated pathway, but whether and how PC could affect the expression and catalytic activity of CYP3A4 via PXR pathway remains unknown. Therefore, in this study, the effect of PC on the CYP3A gene expression was investigated in mice primary hepatocytes after knockdown of PXR by transient transfection of PXR siRNA, and the gene expression, protein expression, and catalytic activity of CYP3A4 in the LS174T cells with PXR overexpression were determined by real-time PCR, western blot analysis, and LC-MS/MS-based CYP3A4 substrate assay, respectively. We found that the level of CYP3a11 gene expression in mouse primary hepatocytes was significantly increased by praeruptorin C, but such an induction was suppressed after knockdown of pregnane X receptor by its siRNA. In PXR-overexpressed LS174T cells, PC significantly enhanced CYP3A4 mRNA, protein expression, and functional activity through PXR-mediated pathway; conversely, no such increase was found in the untransfected cells. These findings suggest that PC can significantly upregulate CYP3A level via the PXR-mediated pathway, and this should be taken into consideration to predict any potential herb-drug interactions between PC, Qianhu, and the other coadministered drugs.

DOI10.1155/2013/156574
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine
WOS SubjectIntegrative & Complementary Medicine
WOS IDWOS:000327982700001
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Sun Yat Sen Univ, Sch Pharmaceut Sci, Lab Drug Metab & Pharmacokinet, Guangzhou 510006, Guangdong, Peoples R China
2.Hainan Med Univ, Sch Pharmaceut Sci, Haikou 571010, Peoples R China
3.Macau Univ, Inst Chinese Med Sci, Macao 999078, Peoples R China
Recommended Citation
GB/T 7714
Huang, Ling,Wu, Qian,Li, Yu-Hua,et al. PXR-Mediated Upregulation of CYP3A Expression by Herb Compound Praeruptorin C from Peucedanum praeruptorum Dunn[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2013.
APA Huang, Ling,Wu, Qian,Li, Yu-Hua,Wang, Yi-Tao,&Bi, Hui-Chang.(2013).PXR-Mediated Upregulation of CYP3A Expression by Herb Compound Praeruptorin C from Peucedanum praeruptorum Dunn.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.
MLA Huang, Ling,et al."PXR-Mediated Upregulation of CYP3A Expression by Herb Compound Praeruptorin C from Peucedanum praeruptorum Dunn".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2013).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Ling]'s Articles
[Wu, Qian]'s Articles
[Li, Yu-Hua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Ling]'s Articles
[Wu, Qian]'s Articles
[Li, Yu-Hua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Ling]'s Articles
[Wu, Qian]'s Articles
[Li, Yu-Hua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.