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Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae
Li, ZH (Li, Zhen Hua)1; Alex, D (Alex, Deepa)1; Siu, SO (Siu, Shiu On)1,2; Chu, IK (Chu, Ivan Keung)2; Renn, J (Renn, Joerg)3; Winkler, C (Winkler, Christoph)3; Lou, S (Lou, Shaoke)4,5; Tsui, SKW (Tsui, Stephen Kwok-Wing)4,5; Zhao, HY (Zhao, Hai Yu)1; Yan, WR (Yan, Wendy Ru)1; Mahady, GB (Mahady, Gail B.)6,7; Li, GH (Li, Guo Hui)2; Kwan, YW (Kwan, Yiu Wa)4; Wang, YT (Wang, Yi Tao)1; Lee, SMY (Lee, Simon Ming-Yuen)1
2011
Source PublicationMOLECULAR BIOSYSTEMS
ISSN1742-206X
Volume7Issue:7Pages:2128-2138
Abstract

Flavonoids isolated from Herba Epimedii such as icaritin, icariin and epimedin C have been suggested as potential bone anabolic compounds. However, the "specific localized effects" of these flavonoids in bone, in vivo, and the metabolism of these flavonoids in zebrafish larvae have never been demonstrated. In this study, we used multiple methods including in vivo imaging, drug metabolites profiling, transcriptomic and proteomic approaches to determine the mechanisms involved in the distribution and metabolism of the flavonoids in zebrafish larvae by measuring the fluorescence emission, in vivo, of icaritin and its glycoside derivatives. The fluorescence emission mechanism of icaritin in vitro was identified by spectrophotometric analysis, and the fluorescent property of icaritin was used as a probe to visualize the metabolism and distribution of icaritin and its glycoside derivatives in zebrafish larvae. Phase I and phase II metabolism of icaritin and its derivatives were identified in zebrafish by mass spectrometry. The combined transcriptomics and proteomics demonstrate a high degree of conservation of phase I and phase II drug metabolic enzymes between zebrafish larvae and mammals. Icaritin and its glycoside derivatives were demonstrated using combined approaches of in vivo imaging, drug metabolites identification, and transcriptomic and proteomic profiling to illustrate phase I and phase II metabolism of the flavonoids and their distribution in bone of zebrafish larvae. This study provides a new methodological model for use of the zebrafish larvae to examine drug metabolism.

KeywordAnimal-models Flavonoids Quercetin Bone Fluorescence Absorption Proteome Swim
DOI10.1039/c1mb00001b
Language英语
WOS IDWOS:000291559900005
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Taipa, Peoples R China
2.Univ Hong Kong, Dept Chem, Pokfulam, Hong Kong, Peoples R China
3.Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore
4.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Hong Kong Bioinformat Ctr, Hong Kong, Hong Kong, Peoples R China
6.Univ Illinois, Coll Pharm, Dept Pan Amer Hlth Org, World Hlth Org Collaborating Ctr Tradit Med, Chicago, IL USA
7.Univ Illinois, Coll Pharm, Dept Pharm Practice, World Hlth Org Collaborating Ctr Tradit Med, Chicago, IL USA
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Li, ZH ,Alex, D ,Siu, SO ,et al. Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae[J]. MOLECULAR BIOSYSTEMS,2011,7(7):2128-2138.
APA Li, ZH .,Alex, D .,Siu, SO .,Chu, IK .,Renn, J .,...&Lee, SMY .(2011).Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae.MOLECULAR BIOSYSTEMS,7(7),2128-2138.
MLA Li, ZH ,et al."Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae".MOLECULAR BIOSYSTEMS 7.7(2011):2128-2138.
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