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Characterization of metabolism of (+)-praeruptorin B and (+)-praeruptorin E in human and rat liver microsomes by liquid chromatography coupled with ion trap mass spectrometry and time-of-flight mass s
Song, YL (Song, Yue-Lin)1; Yan, R (Yan, Ru)1; Jing, WH (Jing, Wang-Hui)1; Zhao, HY (Zhao, Hai-Yu)1; Wang, YT (Wang, Yi-Tao)1,2
2011-04-30
Source PublicationRAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN0951-4198
Volume25Issue:6Pages:719-730
Abstract

Peucedani Radix is a Chinese medicinal herb noted for its effects on treatments of respiratory and pulmonary disorders. As a part of a systematic pharmacokinetic evaluation of the herb in our laboratory, the present study investigated, for the first time, the metabolic profile of (+)-praeruptorin B (dPB) and (+)-praeruptorin E (dPE), two main bioactive constituents of Peucedani Radix in pooled liver microsomes of rats (RLMs) and humans (HLMs). dPE was eliminated faster than dPB in both species. The incubation of dPB with RLMs and HLMs resulted in eight (B1-B8) and nine (B1-B9) metabolites, respectively, while both RLMs and HLMs converted dPE into 13 metabolites (E1-13). Structures of all the metabolites were proposed through comparing their mass data obtained via tandem mass spectrometry on an MSD ion trap system (IT-MS/MS) coupled with high-resolution mass measurement by time-of-flight mass spectrometry (TOF-MS) with those of the respective parent compound. B1 and E1 were unambiguously identified as (-)-cis-khellactone. The formations of all the metabolites were NADPH-dependent. Oxidation and hydrolysis were demonstrated to be two predominant metabolic pathways of dPB and dPE. Oxidation initiated at either the C-3' or C-4' substituent, while hydrolysis only started from the C-3' substituent. Fragmentation of all metabolites followed similar pathways to those of the parent pyranocoumarins. The information on metabolic properties of dPB and dPE and the mass fragmentation profiles of their metabolites obtained in the present study will aid in characterization of metabolic profiles of other angular-type pyranocoumarins and further investigation of in vivo fates of these pyranocoumarins and the herb. Copyright (C) 2011 John Wiley & Sons, Ltd.

DOI10.1002/rcm.4922
Language英语
WOS IDWOS:000287596300006
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China
2.Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Song, YL ,Yan, R ,Jing, WH ,et al. Characterization of metabolism of (+)-praeruptorin B and (+)-praeruptorin E in human and rat liver microsomes by liquid chromatography coupled with ion trap mass spectrometry and time-of-flight mass s[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2011,25(6):719-730.
APA Song, YL ,Yan, R ,Jing, WH ,Zhao, HY ,&Wang, YT .(2011).Characterization of metabolism of (+)-praeruptorin B and (+)-praeruptorin E in human and rat liver microsomes by liquid chromatography coupled with ion trap mass spectrometry and time-of-flight mass s.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,25(6),719-730.
MLA Song, YL ,et al."Characterization of metabolism of (+)-praeruptorin B and (+)-praeruptorin E in human and rat liver microsomes by liquid chromatography coupled with ion trap mass spectrometry and time-of-flight mass s".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 25.6(2011):719-730.
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